Development and Application of a High-Throughput Microbial Clone Picking Workstation Based on Machine Vision Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-038

Fig. 2 System composition of clone selection workstation

Extracts from the Article

克隆挑选工作站整体可分为成像子系统、挑取子系统、清洁子系统、物料传递子系统、环境控制子系统、软件控制系统等6个部分，如图2所示。

4. 以下是市面上可见的几款挑克隆工作站的对比，通过数据对比表明本研究设计的挑克隆工作站，在图像分辨率，菌落识别准确度，挑取准确率各方面已经达到国外产品QPix的相近水平（表2）。

Other Images/Table from this Article

• Fig. 1  Overall form of clone selection workstation
• Fig. 3  Electrical schematic diagram
• Fig. 4  Work area arrangement of clone selection workstation
• Fig. 5  Problems existing in colony images that affect recognition
• Fig. 6  Flowchart of colony identification and location
• Fig. 7  Petri dish
• Fig. 8  Dish trainning curve
• Fig. 9  Identification and labeling of Petri dishes
• Table 1  Distribution of the dataset
• Fig. 10  Colony-training curve
• Fig. 11  Grayscale image generated by reasoning
• Fig. 12  Identification of qualified colonies
• Fig. 13  UI design
• Fig. 14  Control interface framework of the workstation
• Fig.15  Clone selection process
• Fig. 16  Image comparison before and after picking
• Fig. 17  Deep well plate containing colony culture medium
• Fig. 18  Verification of sterilization effect
• Table 2  A comparison of technical parameters of this device with other three cloning selection workstations