Development and Application of a High-Throughput Microbial Clone Picking Workstation Based on Machine Vision Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-038

Fig.15 Clone selection process

Extracts from the Article

实验以完成对生长有酵母菌落的培养皿进行96个克隆挑取为任务目标，在系统软件中建立典型菌落图像识别-挑取-接种-清洁高温灭菌实验流程任务序列。系统初始化完成后，首先将培养皿放置在挑取板位上，对培养皿进行图像采集和图像分析，然后使用挑针阵列完成96个克隆挑取，接种至预加注1000 μL培养液（常规YPD培养基）的深孔板中，最后挑针阵列先后进行2次超声清洁和高温灭菌，流程结束。其中，单次超声清洁时间为5 s，高温灭菌烘干时间为80 s。完整克隆挑选流程如图15所示。

Other Images/Table from this Article

• Fig. 1  Overall form of clone selection workstation
• Fig. 2  System composition of clone selection workstation
• Fig. 3  Electrical schematic diagram
• Fig. 4  Work area arrangement of clone selection workstation
• Fig. 5  Problems existing in colony images that affect recognition
• Fig. 6  Flowchart of colony identification and location
• Fig. 7  Petri dish
• Fig. 8  Dish trainning curve
• Fig. 9  Identification and labeling of Petri dishes
• Table 1  Distribution of the dataset
• Fig. 10  Colony-training curve
• Fig. 11  Grayscale image generated by reasoning
• Fig. 12  Identification of qualified colonies
• Fig. 13  UI design
• Fig. 14  Control interface framework of the workstation
• Fig. 16  Image comparison before and after picking
• Fig. 17  Deep well plate containing colony culture medium
• Fig. 18  Verification of sterilization effect
• Table 2  A comparison of technical parameters of this device with other three cloning selection workstations