Development and Application of a High-Throughput Microbial Clone Picking Workstation Based on Machine Vision Synthetic Biology Journal    DOI: 10.12211/2096-8280.2025-038

Fig. 18 Verification of sterilization effect

Extracts from the Article

此外，还开展了灭菌效果验证实验，分为实验组和对照组，实验组挑针未做清洗灭菌，直接接种至加有培养基的深孔板中。作为对照组，先后使用2个分别盛装有1%次氯酸钠和75%酒精的超声波清洗槽进行预清洁，再使用卤素灯进行高温灭菌和挑针烘干。待挑针冷却后，再次接种至加注有培养基的深孔板中，密封后与实验组同步进行培养。实验中，实验组检出浑浊物，对照组未检出浑浊，证明了所采用的清洁方式和灭菌时长的有效性。实验结果如图18所示。

Other Images/Table from this Article

• Fig. 1  Overall form of clone selection workstation
• Fig. 2  System composition of clone selection workstation
• Fig. 3  Electrical schematic diagram
• Fig. 4  Work area arrangement of clone selection workstation
• Fig. 5  Problems existing in colony images that affect recognition
• Fig. 6  Flowchart of colony identification and location
• Fig. 7  Petri dish
• Fig. 8  Dish trainning curve
• Fig. 9  Identification and labeling of Petri dishes
• Table 1  Distribution of the dataset
• Fig. 10  Colony-training curve
• Fig. 11  Grayscale image generated by reasoning
• Fig. 12  Identification of qualified colonies
• Fig. 13  UI design
• Fig. 14  Control interface framework of the workstation
• Fig.15  Clone selection process
• Fig. 16  Image comparison before and after picking
• Fig. 17  Deep well plate containing colony culture medium
• Table 2  A comparison of technical parameters of this device with other three cloning selection workstations