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中文
IMAGE/TABLE DETAILS
Development and Application of a High-Throughput Microbial Clone Picking Workstation Based on Machine Vision
ZHANG Jiankang, WANG Wenjun, GUO Hong-ju, BAI Beichen, ZHANG Yafei, YUAN Zheng, LI Yanhui, LI Hang
Synthetic Biology Journal
DOI:
10.12211/2096-8280.2025-038
设备
型号
通道
数量
挑取
方式
CCD
分辨率(Pixel/mm)
菌落识别尺寸
(mm)
菌落识别准确度
(%)
挑取准确率
(%)
有效
像素
(万)
QPix 420
96
挑针
22
≥ 0.1
0.5-0.7mm菌落,>97%
> 98%
500
Hedylax T200智能微生物菌落挑选工作站
单通道或8通道
吸头
/
/
/
≥
98%
2500
全自动菌落挑选工作站G3000
2或4
挑针
/
≥ 0.5
/
1 mm以上98%
600-2000
本设备
96
挑针
30
≥0.2
≥
98%
≥
98%
1000
Table 2
A comparison of technical parameters of this device with other three cloning selection workstations
Extracts from the Article
4. 以下是市面上可见的几款挑克隆工作站的对比,通过数据对比表明本研究设计的挑克隆工作站,在图像分辨率,菌落识别准确度,挑取准确率各方面已经达到国外产品QPix的相近水平(表2)。
Other Images/Table from this Article
Fig. 1
Overall form of clone selection workstation
Fig. 2
System composition of clone selection workstation
Fig. 3
Electrical schematic diagram
Fig. 4
Work area arrangement of clone selection workstation
Fig. 5
Problems existing in colony images that affect recognition
Fig. 6
Flowchart of colony identification and location
Fig. 7
Petri dish
Fig. 8
Dish trainning curve
Fig. 9
Identification and labeling of Petri dishes
Table 1 Distribution of the dataset
Fig. 10
Colony-training curve
Fig. 11
Grayscale image generated by reasoning
Fig. 12
Identification of qualified colonies
Fig. 13
UI design
Fig. 14
Control interface framework of the workstation
Fig.15
Clone selection process
Fig. 16
Image comparison before and after picking
Fig. 17
Deep well plate containing colony culture medium
Fig. 18
Verification of sterilization effect