作物光合作用合成生物学的策略与展望

doi:10.12211/2096-8280.2024-094

摘要/Abstract

摘要：

光合作用是地球上几乎所有生命活动的能量和物质来源，其效率直接影响作物的生长和产量。随着合成生物学的快速发展，研究者们开始探索通过工程化手段，从不同层次优化光合作用的基本环节，包括光能利用、碳固定、光呼吸及光合逆境适应等。本文综述了近年来在提高光合作用效率方面的研究进展，重点讨论了新型光能转化模型的构建、Rubisco的定向进化与活性改造、碳同化途径的优化、光呼吸支路的设计以及逆境高光效回路的构建等策略。通过合成生物学的手段，可以显著提高植物的光合效率和抗逆能力，实现生物量和作物产量的提升，为应对全球粮食安全挑战提供新的解决方案。未来，基于合成生物学的策略，深入解析光合作用的分子机制，结合人工智能等新兴技术，将为光合作用的工程化改造提供更为有效的方法和途径，实现作物光合作用效率的显著提升。

关键词: 光合作用效率, 合成生物学, 光合电子传递模型, Rubisco改造, 光呼吸支路, 光合逆境适应

Abstract:

Photosynthesis is the primary source of energy and materials for nearly all life activities on Earth, and its efficiency directly affects crop growth and yield. With the rapid development of synthetic biology, researchers have begun to explore engineering approaches to optimize the fundamental processes of photosynthesis at different levels, such as light energy utilization, carbon fixation, photorespiration, and stress adaptation. This review summarizes recent advances in improving photosynthetic efficiency, with a focus on the synthetic biological strategies that can be implemented in crops. To achieve efficient light absorption and electron transport, novel light energy conversion models have been developed, involving the engineering of light-harvesting antennae to minimize energy loss and the development of orthogonal electron transport chains to enhance quantum yield. Multi-level optimization strategies have been developed for carbon assimilation pathways, including directed evolution and activity modification of Rubisco, optimization of key enzymes in the Calvin-Benson-Bassham cycle, and the introduction of CO₂ concentrating mechanisms into C₃ plants. Furthermore, novel photorespiratory bypasses have been engineered through synthetic biology approaches, which optimize glycolate metabolism to effectively reduce photorespiratory carbon loss while enhancing photosynthetic efficiency in crops. Additionally, various engineering strategies have been developed to optimize photosynthetic performance under adverse conditions, such as the enhancement of non-photochemical quenching components to tolerate high light and the application of stress-responsive elements to adapt to temperature fluctuations. By employing synthetic biology techniques, significant improvements in plant photosynthetic efficiency and stress resistance have been achieved. This has led to enhanced biomass and crop yields, thereby providing new solutions to address global food security challenges. In the future, strategies based on synthetic biology, combined with a deeper understanding of the molecular mechanisms of photosynthesis and emerging technologies like artificial intelligence, will offer more effective methods and pathways for the engineering of photosynthesis, resulting in a substantial enhancement of crop photosynthetic efficiency.

Key words: photosynthetic efficiency, synthetic biology, photosynthetic electron transfer model, Rubisco engineering, photorespiratory bypass, photosynthetic stress adaptation

中图分类号:

Q812

孙扬, 陈立超, 石艳云, 王珂, 吕丹丹, 徐秀美, 张立新. 作物光合作用合成生物学的策略与展望[J]. 合成生物学, DOI: 10.12211/2096-8280.2024-094.

Yang SUN, Lichao CHEN, Yanyun SHI, Ke WANG, Dandan LU, Xiumei XU, Lixin ZHANG. Strategies and prospects of synthetic biology in crop photosynthesis[J]. Synthetic Biology Journal, DOI: 10.12211/2096-8280.2024-094.

图/表 3

图1 光合电子传递的改造和新型光能转化模型的设计（上部分为在现有光合膜系统上开展的电子传递的改造，黄色字体标注目前已开展实验改造的靶点蛋白，橙色虚线箭头和绿色虚线箭头分别标注线式电子传递路径和环式电子传递路径。下部分黑色虚线框内为目前设计（待实验验证）的新型光能转化模型/方案，从左至右依次为Ort等设计的新型光反应中心和电子传递模型［19］，Leister设计的新型捕光模型［11］，以及在高等植物光系统中引入叶绿素f的方案［14］，紫色虚线箭头标注可能的电子传递路径。）

Fig. 1 Engineering of Photosynthetic Electron Transport and Design of Novel Light-Energy Conversion Models(The upper section illustrates modifications of electron transport on the existing photosynthetic membrane system, with target proteins that have been experimentally modified highlighted in yellow. Linear and cyclic electron transport are indicated by orange and green dashed arrows, respectively. The lower section, enclosed within a black dashed box, depicts novel light energy conversion models/projects currently under design (awaiting experimental validation). From left to right, these include: (1) the new photosynthetic reaction center and electron transfer model designed by Ort et al. [19], (2) the novel light-harvesting model proposed by Leister [11], and (3) the introduction of chlorophyll f into the photosystems of higher plants [14]. Purple dashed arrows indicate potential electron transport pathways.)

图2 多层次优化碳同化途径的策略与方法

Fig. 2 Strategies and Technologies for Multi-level Optimization of Carbon Assimilation Pathways

图3 目前已在水稻中构建的光呼吸支路（在水稻中构建的光呼吸支路（黄色字体）将甘油酸（glycolate）直接在叶绿体内代谢（黑色直线箭头），旨在减少光呼吸（Photorespiration）导致的碳损耗，有助于提升CBB循环（CBB Cycle）的固碳效率。）

Fig. 3 Current photorespiratory bypasses constructed in rice.(The photorespiratory bypasses engineered in rice (highlighted in yellow) directly metabolize glycolate within chloroplasts (indicated by black solid arrows), aiming to reduce carbon loss associated with photorespiration and thereby enhance the carbon fixation efficiency of the CBB cycle.)

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