关键词: 群体感应, 种内细胞间通讯, 种间细胞间通讯, 基因线路, 合成生物学

Abstract:

Quorum sensing (QS) is a bacterial cell-to-cell communication system. Bacteria sense the density of bacterial population by secreting diffuse small molecular signals, thus causing the coordinated expression of a group of specific genes at the transcriptional level. With continuous research, the quorum-sensing related genetic elements and the regulatory principles have gradually become clear. In recent years, genetic circuits containing components of the bacterial QS system have been constructed through synthetic biology to achieve intra-species and inter-species artificial communication, and these genetic circuits based on QS have great application potential in biotechnology and biomedicine. This paper reviews several relatively clear and representative microbial quorum sensing systems and their functional roles, and introduces the application of genetic circuits based on quorum sensing systems to intra-species and inter-species cell communication, and discusses the microbial quorum sensing system in constructing biological computing tools, regulating the population density and the flow of metabolism of the future development prospect. For intra-cell communication, we mainly introduced the applications of quorum sensing system in the construction of biological computing tools, which are mainly reflected in the research of toggle switches, biosensors and logic gates in synthetic biology. Toggle switches, biosensors, and logic gates designed based on quorum sensing mechanism can better coordinate cell behavior at the population level by combining with some biological control circuits to achieve precise regulation at the spatial, temporal, and population level. For inter-species cell communication, we mainly discuss the influence of introducing quorum sensing system on controlling population density and regulating metabolic flow. Quorum sensing system is used to redistribute the metabolic fluxes of the desired pathways through the recombination of metabolic network, to realize the regulation of population density and co-culture of mixed strains. Meanwhile, it is also found that the combination of QS system and oscillator model has great potential in regulating the synchronicity of microbial complexes. In summary, the in-depth research on the quorum sensing mechanism and application not only lays a certain foundation for elucidating the mechanism of microbial ecological competition and dynamic balance, but also provides an idea for clarifying the regulation mechanism of pathogenic bacteria and developing new disease control strategies.

Key words: quorum sensing, intra-species cell communication, inter-species cell communication, genetic circuits, synthetic biology