合成生物学 ›› 2020, Vol. 1 ›› Issue (5): 570-582.DOI: 10.12211/2096-8280.2020-038
刘美霞1,2, 李强子1,2, 孟冬冬2, 魏欣蕾2, 游淳1,2
收稿日期:
2020-04-03
修回日期:
2020-09-24
出版日期:
2020-10-31
发布日期:
2020-12-03
通讯作者:
游淳
作者简介:
作者简介:刘美霞(1990—),女,博士研究生。研究方向为生物化工。E-mail:基金资助:
Meixia LIU1,2, Qiangzi LI1,2, Dongdong MENG2, Xinlei WEI2, Chun YOU1,2
Received:
2020-04-03
Revised:
2020-09-24
Online:
2020-10-31
Published:
2020-12-03
Contact:
Chun YOU
摘要:
烟酰胺类辅酶NAD(P)是生命体氧化还原过程中最常见的电子中介体。绝大多数氧化还原酶都需要NAD或NADP进行分解代谢和合成代谢,但根据其在代谢过程中的作用,不同氧化还原酶表现出不同的辅酶偏好性。为了解决辅酶在代谢过程中的供需平衡、生产成本和稳定性等问题,对氧化还原酶的辅酶偏好性改造是蛋白质工程的研究热点。本文综述了氧化还原酶的天然烟酰胺辅酶偏好性的改造方法和研究进展,以及天然辅酶偏好性改造工程在提高产品收率和降低生产成本方面的应用。仿生烟酰胺辅酶因成本低、稳定性高,常被用来代替天然烟酰胺辅酶参与氧化还原反应,因此本文还详细介绍了改造酶元件使其偏好仿生烟酰胺辅酶以及其在合成生物学领域构建体内生物正交氧化还原途径和体外合成途径等方面的研究进展。随着更多天然酶元件以及新型优良仿生辅酶的挖掘和设计,从天然辅酶到仿生辅酶的辅酶偏好性改造已成为辅酶工程的一个重要方向。
中图分类号:
刘美霞, 李强子, 孟冬冬, 魏欣蕾, 游淳. 烟酰胺类辅酶依赖型氧化还原酶的辅酶偏好性改造及其在合成生物学中的应用[J]. 合成生物学, 2020, 1(5): 570-582.
Meixia LIU, Qiangzi LI, Dongdong MENG, Xinlei WEI, Chun YOU. Protein engineering of nicotinamide coenzyme-dependent oxidoreductases for coenzyme preference and its application in synthetic biology[J]. Synthetic Biology Journal, 2020, 1(5): 570-582.
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