Synthetic Biology Journal ›› 2022, Vol. 3 ›› Issue (2): 369-384.DOI: 10.12211/2096-8280.2021-060
• Invited Review • Previous Articles Next Articles
WU Weihong1, LI Wei2, ZHANG Xian’en3, CUI Zongqiang2
Received:
2021-05-13
Revised:
2021-09-06
Online:
2022-05-11
Published:
2022-04-30
Contact:
CUI Zongqiang
武伟红1, 李炜2, 张先恩3, 崔宗强2
通讯作者:
崔宗强
作者简介:
基金资助:
CLC Number:
WU Weihong, LI Wei, ZHANG Xian’en, CUI Zongqiang. Synthetic biology for fluorescent bioimaging[J]. Synthetic Biology Journal, 2022, 3(2): 369-384.
武伟红, 李炜, 张先恩, 崔宗强. 合成生物学与荧光成像技术[J]. 合成生物学, 2022, 3(2): 369-384.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2021-060
荧光材料 | 大小 | 荧光强度 | 荧光量子效率 | 荧光寿命 |
---|---|---|---|---|
量子点 | 2~20 nm | +++++ | 0.1~0.85 | 20 min |
荧光蛋白 | 4 nm | + | 0.79 | 100 ms |
荧光RNA | 40 bp | +++ | 0.30~0.70 | > 15 min |
Tab. 1 Properties of different fluorescent materials [74-76]
荧光材料 | 大小 | 荧光强度 | 荧光量子效率 | 荧光寿命 |
---|---|---|---|---|
量子点 | 2~20 nm | +++++ | 0.1~0.85 | 20 min |
荧光蛋白 | 4 nm | + | 0.79 | 100 ms |
荧光RNA | 40 bp | +++ | 0.30~0.70 | > 15 min |
Fig. 5 Schematic diagram for imaging polymerase activity in single cells through aptamer corn fluorophore[87](HEK293T cells transformed with the reporter plasmid could produce yellow fluorescence by adding fluorophore DFHO, but the fluorescence disappears after adding the RNA polymerase inhibitor actinomycin D.)
Fig. 7 Schematic diagram for imaging of the fluorescent RNA complex in HeLa cells[76](A series of analogues of synthetic dye HBC combined with Pepper can emit fluorescence with different colors. HBC analogues were combined with Pepper in HeLa cells and are compared with mock cells. Imaging observation is carried out with confocal microscope and two-photon microscope.)
Fig. 9 Real-time dynamic tracking of the influenza virus unpacking process[75][Under electron microscope, QD625 is black and round, while QD705 is triangular. vRNP is labeled with QD625 (red) and QD705 (green) respectively, and the results showed that QD625 and QD705 are released during the hulling process, respectively. IAV-released vRNPs released from IAV reach the nucleus through a three-stage active transport, where they undergo two diffusion modes.]
Fig. 11 SV40-QD VLPs for imaging atherosclerotic plaques in targeted mice[100](SV40 VLPs contain atherosclerotic targeting units, thrombin inhibitors, which can encapsulate as QD800. FH-QDS can target atherosclerotic areas in mice.)
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