Yongxiang SONG1,2,3, Xiufeng ZHANG1,2,3, Yanqin LI1,2,3, Hua XIAO1,2,3, Yan YAN1,2,3
Received:
2023-12-01
Revised:
2024-03-08
Published:
2024-03-14
Contact:
Yan YAN
宋永相1,2,3, 张秀凤1,2,3, 李艳芹1,2,3, 肖华1,2,3, 闫岩1,2,3
通讯作者:
闫岩
作者简介:
基金资助:
CLC Number:
Yongxiang SONG, Xiufeng ZHANG, Yanqin LI, Hua XIAO, Yan YAN. Resistance-gene directed discovery of bioactive natural products[J]. Synthetic Biology Journal, DOI: 10.12211/2096-8280.2023-099.
宋永相, 张秀凤, 李艳芹, 肖华, 闫岩. 自抗性基因导向的活性天然产物挖掘[J]. 合成生物学, DOI: 10.12211/2096-8280.2023-099.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2023-099
Fig. 3 Self-resistance-gene directed localization of natural product biosynthetic gene clusters (BGCs) with a known target: the biosynthetic core genes are shown in blue, self-resistance genes in red, and other related genes in grey
Fig. 4 Determination of the target of natural products through resistance genes: biosynthetic core genes are shown in blue, self-resistance genes in red, and other related genes in grey
Fig. 5 Workflow of resistance gene directed dereplication of natural products: (1) Construction of vectors expressing a known antibiotic resistance gene; (2) Construction of strains capable of indicating the presence of known bioactive compounds; (3) Construction of a library of indicator strains; (4) Inoculation of the indicator strains to media containing bioactive natural products; (5) Identification of natural products with new modes of action; (6) Isolation of natural products with new modes of action
Fig. 6 Discovery of bioactive natural products guided by resistance genes: biosynthetic core genes are shown in blue, self-resistance genes in red, and other related genes in grey
Fig. 7 The "false positive" resistance genes in biosynthetic gene clusters of natural products: biosynthetic core genes are shown in blue, self-resistance genes in red, and other related genes in grey
Fig. 8 Discovery of new antibiotics guided by resistance gene phylogeny: the glycopeptide self-resistance determinants of complestatin and corbomycin are distinctive to known determinant vanY and vanHAX among 71 glycopeptide BGCs
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