Synthetic Biology Journal ›› 2020, Vol. 1 ›› Issue (2): 158-173.DOI: 10.12211/2096-8280.2020-017
• Invited Review • Previous Articles Next Articles
Jiaoqi GAO1,2, Yongjin ZHOU1,2
Received:
2020-03-05
Revised:
2020-03-23
Online:
2020-08-04
Published:
2020-04-30
高教琪1,2, 周雍进1,2
作者简介:
高教琪(1989—),男,博士,助理研究员,主要从事多形汉逊酵母甲醇生物转化及产物合成研究。E-mail:基金资助:
CLC Number:
Jiaoqi GAO, Yongjin ZHOU. Advances in methanol bio-transformation[J]. Synthetic Biology Journal, 2020, 1(2): 158-173.
高教琪, 周雍进. 甲醇生物转化的机遇与挑战[J]. 合成生物学, 2020, 1(2): 158-173.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2020-017
1 | DALENA F, SENATORE A, BASILE M, et al. Advances in methanol production and utilization, with particular emphasis toward hydrogen generation via membrane reactor technology[J]. Membranes, 2018, 8(4): 98. |
2 | OLAH G A. Beyond oil and gas: the methanol economy[J]. Angewandte Chemie International Edition, 2005, 44(18): 2636-2639. |
3 | TIAN P, WEI Y, YE M, et al. Methanol to olefins (MTO): from fundamentals to commercialization[J]. ACS Catalysis, 2015, 5(3): 1922-1938. |
4 | ZENG A P. New bioproduction systems for chemicals and fuels: needs and new development[J]. Biotechnology Advances, 2019, 37: 11. |
5 | PFEIFENSCHNEIDER J, BRAUTASET T, WENDISCH V F. Methanol as carbon substrate in the bio‐economy: metabolic engineering of aerobic methylotrophic bacteria for production of value‐added chemicals[J]. Biofuels, Bioproducts and Biorefining, 2017, 11(4): 719-731. |
6 | BOZZANO G, MANENTI F. Efficient methanol synthesis: Perspectives, technologies and optimization strategies[J]. Progress in Energy and Combustion Science, 2016, 56: 71-105. |
7 | ZHOU Y J, KERKHOVEN E J, NIELSEN J. Barriers and opportunities in bio-based production of hydrocarbons[J]. Nature Energy, 2018, 3(11): 925-935. |
8 | SCHRADER J, SCHILLING M, HOLTMANN D, et al. Methanol-based industrial biotechnology: current status and future perspectives of methylotrophic bacteria[J]. Trends in Biotechnology, 2009, 27(2): 107-115. |
9 | OLAH G A. Towards oil independence through renewable methanol chemistry[J]. Angewandte Chemie International Edition, 2013, 52(1): 104-107. |
10 | KNöZINGER H, WEITKAMP J. Handbook of heterogeneous catalysis [M]. New York:Wiley-VCH, 1997. |
11 | BERTAU M, OFFERMANNS H, PLASS L, et al. Methanol: the basic chemical and energy feedstock of the future[M]. Berlin:Springer, 2014. |
12 | YANG Y, XU J, LIU Z, et al. Progress in coal chemical technologies of China[J]. Reviews in Chemical Engineering, 2019, 36(1): 21-66. |
13 | CHEJNE F, HERNANDEZ J. Modelling and simulation of coal gasification process in fluidised bed[J]. Fuel, 2002, 81(13): 1687-1702. |
14 | DAI J, SAAYMAN J, GRACE J R, et al. Gasification of woody biomass[J]. Annual Review of Chemical and Biomolecular Engineering, 2015, 6: 77-99. |
15 | TROP P, ANICIC B, GORICANEC D. Production of methanol from a mixture of torrefied biomass and coal[J]. Energy, 2014, 77: 125-132. |
16 | ZHANG X, ZHONG L, GUO Q, et al. Influence of the calcination on the activity and stability of the Cu/ZnO/Al2O3 catalyst in liquid phase methanol synthesis[J]. Fuel, 2010, 89(7): 1348-1352. |
17 | 石磊, 张婉莹, 王玉鑫. 低温甲醇合成研究进展[J]. 化工学报, 2015, 66(9): 3333-3340. |
SHI L, ZHANG W Y, WANG Y X. Research developments of low-temperature methanol synthesis[J]. CIESC Journal, 2015, 66(9): 3333-3340. | |
18 | SILVA M J DA. Synthesis of methanol from methane: Challenges and advances on the multi-step (syngas) and one-step routes (DMTM)[J]. Fuel Processing Technology, 2016, 145:42-61. |
19 | HUANG W, ZHANG S, TANG Y, et al. Low‐temperature transformation of methane to methanol on Pd1O4 single sites anchored on the internal surface of microporous silicate[J]. Angewandte Chemie International Edition, 2016, 55(43): 13441-13445. |
20 | GRUNDNER S, MARKOVITS M A, LI G, et al. Single-site trinuclear copper oxygen clusters in mordenite for selective conversion of methane to methanol[J]. Nature Communications, 2015, 6: 7546. |
21 | JIN Z, WANG L, ZUIDEMA E, et al. Hydrophobic zeolite modification for in situ peroxide formation in methane oxidation to methanol[J]. Science, 2020, 367(6474): 193-197. |
22 | SAZINSKY M H, LIPPARD S J. Methane monooxygenase: functionalizing methane at iron and copper[M]. Berlin: Springer, 2015: 205-256. |
23 | HWANG I Y, LEE S H, CHOI Y S, et al. Biocatalytic conversion of methane to methanol as a key step for development of methane-based biorefineries[J]. Journal of Microbiology and Biotechnology, 2014, 24(12): 1597-1605. |
24 | DUAN C, LUO M, XING X. High-rate conversion of methane to methanol by Methylosinus trichosporium OB3b[J]. Bioresource Technology, 2011, 102(15): 7349-7353. |
25 | KAHN B. The world passes 400 PPM threshold. Permanently[R]. Princeton: Climate Central, 2016. |
26 | LI W, WANG H, JIANG X, et al. A short review of recent advances in CO2 hydrogenation to hydrocarbons over heterogeneous catalysts[J]. RSC Advances, 2018, 8(14): 7651-7669. |
27 | NAVARRO R, SANCHEZ-SANCHEZ M, ALVAREZ-GALVAN M, et al. Hydrogen production from renewable sources: biomass and photocatalytic opportunities[J]. Energy & Environmental Science, 2009, 2(1): 35-54. |
28 | YOON Y, HALL A S, SURENDRANATH Y. Tuning of silver catalyst mesostructure promotes selective carbon dioxide conversion into fuels[J]. Angewandte Chemie International Edition, 2016, 55(49): 15282-15286. |
29 | LI K, PENG B, PENG T. Recent advances in heterogeneous photocatalytic CO2 conversion to solar fuels[J]. ACS Catalysis, 2016, 6(11): 43. |
30 | KATTEL S, RAMíREZ P J, CHEN J G, et al. Active sites for CO2 hydrogenation to methanol on Cu/ZnO catalysts[J]. Science, 2017, 355(6331): 1296-1299. |
31 | RUI N, WANG Z, SUN K, et al. CO2 hydrogenation to methanol over Pd/In2O3: effects of Pd and oxygen vacancy[J]. Applied Catalysis B: Environmental, 2017, 218: 488-497. |
32 | SUN K, FAN Z, YE J, et al. Hydrogenation of CO2 to methanol over In2O3 catalyst[J]. Journal of CO2 Utilization, 2015, 12: 1-6. |
33 | LI H, WANG L, DAI Y, et al. Synergetic interaction between neighbouring platinum monomers in CO2 hydrogenation[J]. Nature Nanotechnology, 2018, 13(5): 411. |
34 | WANG Y, KATTEL S, GAO W, et al. Exploring the ternary interactions in Cu-ZnO-ZrO2 catalysts for efficient CO2 hydrogenation to methanol[J]. Nature Communications, 2019, 10(1): 1166. |
35 | SHIH C F, ZHANG T, LI J, et al. Powering the future with liquid sunshine[J]. Joule, 2018, 2(10): 1925-1949. |
36 | WITTHOFF S, MüHLROTH A, MARIENHAGEN J, et al. C1 metabolism in Corynebacterium glutamicum: an endogenous pathway for oxidation of methanol to carbon dioxide[J]. Applied and Environmental Microbiology, 2013, 79(22): 6974-6983. |
37 | KROG A, HEGGESET T M, MüLLER J E, et al. Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties[J]. PLoS One, 2013, 8(3): E59188. |
38 | HEGGESET T M, KROG A, BALZER S, et al. Genome sequence of thermotolerant Bacillus methanolicus: features and regulation related to methylotrophy and production of L-lysine and L-glutamate from methanol[J]. Applied and Environmental Microbiology, 2012, 78(15): 5170-5181. |
39 | BRAUTASET T, JAKOBSEN Ø M, DEGNES K F, et al. Bacillus methanolicus pyruvate carboxylase and homoserine dehydrogenase I and II and their roles for L-lysine production from methanol at 50oC[J]. Applied Microbiology and Biotechnology, 2010, 87(3): 951-964. |
40 | IRLA M, NÆRDAL I, BRAUTASET T, et al. Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains[J]. Industrial Crops and Products, 2017, 106: 12-20. |
41 | IRLA M, HEGGESET T, NAERDAL I, et al. Genome-based genetic tool development for Bacillus methanolicus: theta-and rolling circle-replicating plasmids for inducible gene expression and application to methanol-based cadaverine production[J]. Frontiers in Microbiology, 2016, 7: 1481. |
42 | CUI L Y, WANG S S, GUAN C G, et al. Breeding of methanol‐tolerant Methylobacterium extorquens AM1 by atmospheric and room temperature plasma mutagenesis combined with adaptive laboratory evolution[J]. Biotechnology Journal, 2018, 13(6): 1700679. |
43 | YANG Y M, CHEN W J, YANG J, et al. Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route[J]. Microbial Cell Factories, 2017, 16(1): 179. |
44 | ROHDE M T, TISCHER S, HARMS H, et al. Production of 2-hydroxyisobutyric acid from methanol by Methylobacterium extorquens AM1 expressing (R)-3-hydroxybutyryl coenzyme A-isomerizing enzymes[J]. Applied and Environmental Microbiology, 2017, 83(3): E02622-E02616. |
45 | HU B, YANG Y M, BECK D A, et al. Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance[J]. Biotechnology for Biofuels, 2016, 9(1): 84. |
46 | SONNTAG F, KRONER C, LUBUTA P, et al. Engineering Methylobacterium extorquens for de novo synthesis of the sesquiterpenoid α-humulene from methanol[J]. Metabolic Engineering, 2015, 32: 82-94. |
47 | LIU Y, TU X, XU Q, et al. Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol[J]. Metabolic Engineering, 2018, 45: 189-199. |
48 | YAMADA R, OGURA K, KIMOTO Y, et al. Toward the construction of a technology platform for chemicals production from methanol: D-lactic acid production from methanol by an engineered yeast Pichia pastoris[J]. World Journal of Microbiology and Biotechnology, 2019, 35(2): 37. |
49 | UBIYVOVK V M, ANANIN V M, MALYSHEV A Y, et al. Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1[J]. BMC Biotechnology, 2011, 11(1): 8. |
50 | OCHSNER A M, SONNTAG F, BUCHHAUPT M, et al. Methylobacterium extorquens: methylotrophy and biotechnological applications[J]. Applied Microbiology and Biotechnology, 2015, 99(2): 517-534. |
51 | RAVIN N V, ELDAROV M A, KADNIKOV V V, et al. Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1[J]. BMC Genomics, 2013, 14(1): 837. |
52 | BRAUTASET T, JAKOBSEN Ø M, FLICKINGER M C, et al. Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicus[J]. Journal of Bacteriology, 2004, 186(5): 1229-1238. |
53 | YU H, LIAO J C. A modified serine cycle in Escherichia coli coverts methanol and CO2 to two-carbon compounds[J]. Nature Communications, 2018, 9(1): 1-10. |
54 | MÜLLER J E, MEYER F, LITSANOV B, et al. Engineering Escherichia coli for methanol conversion[J]. Metabolic Engineering, 2015, 28: 190-201. |
55 | GREEN P N, ARDLEY J K. Review of the genus Methylobacterium and closely related organisms: a proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov[J]. International Journal of Systematic and Evolutionary Microbiology, 2018, 68(9): 2727-2748. |
56 | ZHAO L, CHANG W C, XIAO Y, et al. Methylerythritol phosphate pathway of isoprenoid biosynthesis[J]. Annual Review of Biochemistry, 2013, 82: 497-530. |
57 | PEÑA D A, GASSER B, ZANGHELLINI J, et al. Metabolic engineering of Pichia pastoris[J]. Metabolic Engineering, 2018, 50: 2-15. |
58 | SCHWARZHANS J P, LUTTERMANN T, GEIER M, et al. Towards systems metabolic engineering in Pichia pastoris[J]. Biotechnology Advances, 2017, 35(6): 681-710. |
59 | YANG Z, ZHANG Z. Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review[J]. Biotechnology Advances, 2018, 36(1): 182-195. |
60 | XUE Y, KONG C, SHEN W, et al. Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway[J]. Journal of Biotechnology, 2017, 242: 64-72. |
61 | 刘爽, 高教琪, 薛闯, 等. 多形汉逊酵母提高生长性能的培养基优化[J]. 生物加工过程, 2020, 18(1): 116-125. |
LIU S, GAO J Q, XUE C, et al. Medium optimization for growth of Ogataea polymorpha[J]. Chinese Journal of Bioprocess Engineering, 2020, 18(1): 116-125. | |
62 | VORONOVSKY A Y, ROHULYA O V, ABBAS C A, et al. Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan[J]. Metabolic Engineering, 2009, 11(4/5): 234-242. |
63 | BOGORAD I W, CHEN C T, THEISEN M K, et al. Building carbon-carbon bonds using a biocatalytic methanol condensation cycle[J]. PNAS, 2014, 111(45): 15928-15933. |
64 | LU X, LIU Y, YANG Y, et al. Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design[J]. Nature Communications, 2019, 10(1): 1-10. |
65 | 陈阳, 杨雪, 袁倩倩, 等. 一碳化合物利用新途径的设计和体外构建[J]. 生物加工过程, 2017, 15(5): 86-92. |
CHEN Y, YANG X, YUAN Q Q, et al. A computationally designed pathway for one carbon compounds utilization and its in vitro construction[J]. Chinese Journal of Bioprocess Engineering, 2017, 15(5): 86-92. | |
66 | CAI P, GAO J, ZHOU Y. CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications[J]. Microbial Cell Factories, 2019, 18(1): 63. |
67 | WANG L, DENG A, ZHANG Y, et al. Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts[J]. Biotechnology for Biofuels, 2018, 11(1): 1-16. |
68 | JUERGENS H, VARELA J A, GORTER DE VRIES A R, et al. Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid[J]. FEMS Yeast Research, 2018, 18(3): foy012. |
69 | NUMAMOTO M, MAEKAWA H, KANEKO Y. Efficient genome editing by CRISPR/Cas9 with a tRNA-sgRNA fusion in the methylotrophic yeast Ogataea polymorpha[J]. Journal of Bioscience and Bioengineering, 2017, 124(5): 487-492. |
70 | WENINGER A, FISCHER J E, RASCHMANOVá H, et al. Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers[J]. Journal of Cellular Biochemistry, 2018, 119(4): 3183-3198. |
71 | WENINGER A, HATZL A M, SCHMID C, et al. Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris[J]. Journal of Biotechnology, 2016, 235: 139-149. |
72 | ZHANG W, ZHANG T, SONG M, et al. Metabolic engineering of Escherichia coli for high yield production of succinic acid driven by methanol[J]. ACS Synthetic Biology, 2018, 7(12): 2803-2811. |
73 | WHITAKER W B, JONES J A, BENNETT R K, et al. Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli[J]. Metabolic Engineering, 2017, 39: 49-59. |
74 | MEYER F, KELLER P, HARTL J, et al. Methanol-essential growth of Escherichia coli[J]. Nature Communications, 2018, 9(1): 1-10. |
75 | TUYISHIME P, WANG Y, FAN L, et al. Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production[J]. Metabolic Engineering, 2018, 49: 220-231. |
76 | LEßMEIER L, PFEIFENSCHNEIDER J, CARNICER M, et al. Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate[J]. Applied Microbiology and Biotechnology, 2015, 99(23): 10163-10176. |
77 | DAI Z, GU H, ZHANG S, et al. Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae[J]. Bioresource Technology, 2017, 245: 1407-1412. |
78 | 周文娟, 刘娇, 李庆刚,等 赖氨酸工业发展的机遇与挑战[J]. 生物产业技术, 2019, 1: 12. |
ZHOU W J, LIU J, LI Q G, et al. Opportunities and challenges in the development of lysine industry[J]. Biotechnology & Business, 2019, 1: 12. | |
79 | BARITUGO K A G, KIM H T, DAVID Y C, et al. Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery[J]. Biofuels, Bioproducts and Biorefining, 2018, 12(5): 899-925. |
80 | 高教琪, 段兴鹏, 周雍进. 酵母细胞工厂生产脂肪酸及其衍生物[J]. 生物加工过程, 2018, 16(1): 19-30. |
GAO J Q, DUAN X P, ZHOU Y J. Production of fatty acids and their derivatives by yeast cell factories[J]. Chinese Journal of Bioprocess Engineering, 2018, 16(1): 19-30. | |
81 | YASOKAWA D, MURATA S, IWAHASHI Y, et al. Toxicity of methanol and formaldehyde towards Saccharomyces cerevisiae as assessed by DNA microarray analysis[J]. Applied Biochemistry and Biotechnology, 2010, 160(6): 1685-1698. |
82 | PRICE J V, CHEN L, WHITAKER W B, et al. Scaffoldless engineered enzyme assembly for enhanced methanol utilization[J]. PNAS, 2016, 113(45): 12691-12696. |
83 | 王千, 程健, 江会锋. 新基因起源: 从自然进化到人工设计[J]. 生物工程学报, 2017, 33(3): 324-330. |
WANG Q, CHENG J, JIANG H F. Origin of new genes: from evolution to design[J]. Chinese Journal of Biotechnology, 2017, 33(3): 324-330. | |
84 | WOOLSTON B M, KING J R, REITER M, et al. Improving formaldehyde consumption drives methanol assimilation in engineered E. coli[J]. Nature Communications, 2018, 9(1): 1-12. |
85 | GONZALEZ J E, BENNETT R K, PAPOUTSAKIS E T, et al. Methanol assimilation in Escherichia coli is improved by coutilization of threonine and deletion of leucine-responsive regulatory protein[J]. Metabolic Engineering, 2018, 45: 67-74. |
86 | CHEN C T, CHEN F Y H, BOGORAD I W, et al. Synthetic methanol auxotrophy of Escherichia coli for methanol-dependent growth and production[J]. Metabolic Engineering, 2018, 49: 257-266. |
87 | BENNETT R K, GONZALEZ J E, WHITAKER W B, et al. Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph[J]. Metabolic Engineering, 2018, 45: 75-85. |
88 | HE H, EDLICH-MUTH C, LINDNER S N, et al. Ribulose monophosphate shunt provides nearly all biomass and energy required for growth of E. coli[J]. ACS Synthetic Biology, 2018, 7(6): 1601-1611. |
89 | ROHLHILL J, SANDOVAL N R, PAPOUTSAKIS E T. Sort-seq approach to engineering a formaldehyde-inducible promoter for dynamically regulated Escherichia coli growth on methanol[J]. ACS Synthetic Biology, 2017, 6(8): 1584-1595. |
90 | HAMMER S K, AVALOS J L. Harnessing yeast organelles for metabolic engineering[J]. Nature Chemical Biology, 2017, 13(8): 823. |
91 | SANDBERG T E, SALAZAR M J, WENG L L, et al. The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology[J]. Metabolic Engineering, 2019, 56: 1-16. |
92 | 曲戈, 赵晶, 郑平, 等 定向进化技术的最新进展[J]. 生物工程学报, 2018, 34(1): 1-11. |
QU G, ZHAO J, ZHENG P, et al. Recent advances in directed evolution[J]. Chinese Journal of Biotechnology, 2018, 34(1): 1-11. |
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