Synthetic Biology Journal ›› 2022, Vol. 3 ›› Issue (5): 901-914.DOI: 10.12211/2096-8280.2021-096
• Invited Review • Previous Articles Next Articles
Quanyu ZHAO
Received:
2021-09-30
Revised:
2021-11-18
Online:
2022-11-16
Published:
2022-10-31
Contact:
Quanyu ZHAO
赵权宇
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赵权宇
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Quanyu ZHAO. Research progress in carbon neutrality oriented adaptive laboratory evolution of microalgae[J]. Synthetic Biology Journal, 2022, 3(5): 901-914.
赵权宇. 面向碳中和的微藻适应性实验室进化研究进展[J]. 合成生物学, 2022, 3(5): 901-914.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2021-096
出发藻株 | 适应进化压力 | 接种细胞密度 | 总时间/d | 文献 |
---|---|---|---|---|
Desmodesmus sp. MAS1, Heterochlorella sp. MAS3 | pH 3.0 | — | 14 | [ |
Chlamydomonas reinhardtii | 20 μmol/(m2·s), 60 μmol/(m2·s), 400 μmol/(m2·s) | — | — | [ |
Coccomyxa sp.M2 | pH 3.0~7.0 | — | — | [ |
Chlorella sp. LAMB 3, Chlorella sp. LAMB 122 | 40% CO2 | 0.4 g/L | 7 | [ |
Chlorellapyrenoidosa | 1500~4750 mg/L NH4+ | 1×108 cells/mL | 19 | [ |
Scenedesmus sp. | 盐浓度0.5%~2.5% 光强4.0~6.0 klux 温度30~35 ℃ | 0.1 g/L 0.1 g/L 0.1 g/L | 10 10 10 | [ |
Chlamydomonas reinhardtii | 0.2 mol/L NaCl | 5×105 cells/mL | 2 | [ |
Tab. 1 Short-term acclimation for microalgae
出发藻株 | 适应进化压力 | 接种细胞密度 | 总时间/d | 文献 |
---|---|---|---|---|
Desmodesmus sp. MAS1, Heterochlorella sp. MAS3 | pH 3.0 | — | 14 | [ |
Chlamydomonas reinhardtii | 20 μmol/(m2·s), 60 μmol/(m2·s), 400 μmol/(m2·s) | — | — | [ |
Coccomyxa sp.M2 | pH 3.0~7.0 | — | — | [ |
Chlorella sp. LAMB 3, Chlorella sp. LAMB 122 | 40% CO2 | 0.4 g/L | 7 | [ |
Chlorellapyrenoidosa | 1500~4750 mg/L NH4+ | 1×108 cells/mL | 19 | [ |
Scenedesmus sp. | 盐浓度0.5%~2.5% 光强4.0~6.0 klux 温度30~35 ℃ | 0.1 g/L 0.1 g/L 0.1 g/L | 10 10 10 | [ |
Chlamydomonas reinhardtii | 0.2 mol/L NaCl | 5×105 cells/mL | 2 | [ |
出发藻株 | 适应进化压力 | 接种细胞密度 /(g/L) | 周期 | 总时间/d | 文献 |
---|---|---|---|---|---|
Chlorococcum littorale | 缺氮 | OD750=0.5 | 13 | 134(前8个周期,每个周期前2天正常,后面6天 缺氮;后面5个周期,前2天正常,后面12天缺氮) | [ |
Chlorella vulgaris | 255 μE/(m2·s)红光 | 0.84 | 38 | 114 | [ |
Dunaliella salina | 42 μE/(m2·s)蓝光 + 128 μE/(m2·s)红光 | 0.5 | 16 | 80 | [ |
Dunaliella salina | 42 μE/(m2·s)蓝光 + 128 μE/(m2·s)红光 + 2.5 mol/L NaCl | — | — | — | [ |
Tisochrysis lutea | 温度波动(低温6~21 ℃, 高温26~35 ℃) | — | 13 | 150 | [ |
Synechocystis sp. PCC 6803 | 3% NaCl | OD730=0.2 | 43 | 303 | [ |
Schizochytrium sp. | 34.5 ℃ | — | 70 | 70 | [ |
Chlamydomonas sp. | 5%或7%海盐 | 0.02 | — | 84 | [ |
Isochrysis galbana Parke | 100 mg/L或200 mg/L苯酚 | 5×105 cells/mL | 30 | 90 | [ |
Crypthecodiniumcohnii | 9~54 g/L葡萄糖 | OD470=0.1 | 260 | 650 | [ |
藻菌共培养 | 体积分数20%渗滤液 | — | 21天一个 周期 | 24个月 | [ |
C.reinhardtii | 0.2 mol/L NaCl | 5×105 cells/mL | 2~3天 一个周期 | 17个月 | [ |
Schizochytrium sp. | 30 g/L NaCl | — | 150 | 150 | [ |
Schizochytrium sp. | 4 ℃, 30 g/L NaCl | — | 30 | 90 | [ |
Schizochytrium sp. | 230 r/min | — | 40 | 40 | [ |
Phaeodactylumtricornutum | 50%~90%盐度 | 1×106 cells/mL | 35 | 252 | [ |
Tab. 2 Long-term ALE for microalgae
出发藻株 | 适应进化压力 | 接种细胞密度 /(g/L) | 周期 | 总时间/d | 文献 |
---|---|---|---|---|---|
Chlorococcum littorale | 缺氮 | OD750=0.5 | 13 | 134(前8个周期,每个周期前2天正常,后面6天 缺氮;后面5个周期,前2天正常,后面12天缺氮) | [ |
Chlorella vulgaris | 255 μE/(m2·s)红光 | 0.84 | 38 | 114 | [ |
Dunaliella salina | 42 μE/(m2·s)蓝光 + 128 μE/(m2·s)红光 | 0.5 | 16 | 80 | [ |
Dunaliella salina | 42 μE/(m2·s)蓝光 + 128 μE/(m2·s)红光 + 2.5 mol/L NaCl | — | — | — | [ |
Tisochrysis lutea | 温度波动(低温6~21 ℃, 高温26~35 ℃) | — | 13 | 150 | [ |
Synechocystis sp. PCC 6803 | 3% NaCl | OD730=0.2 | 43 | 303 | [ |
Schizochytrium sp. | 34.5 ℃ | — | 70 | 70 | [ |
Chlamydomonas sp. | 5%或7%海盐 | 0.02 | — | 84 | [ |
Isochrysis galbana Parke | 100 mg/L或200 mg/L苯酚 | 5×105 cells/mL | 30 | 90 | [ |
Crypthecodiniumcohnii | 9~54 g/L葡萄糖 | OD470=0.1 | 260 | 650 | [ |
藻菌共培养 | 体积分数20%渗滤液 | — | 21天一个 周期 | 24个月 | [ |
C.reinhardtii | 0.2 mol/L NaCl | 5×105 cells/mL | 2~3天 一个周期 | 17个月 | [ |
Schizochytrium sp. | 30 g/L NaCl | — | 150 | 150 | [ |
Schizochytrium sp. | 4 ℃, 30 g/L NaCl | — | 30 | 90 | [ |
Schizochytrium sp. | 230 r/min | — | 40 | 40 | [ |
Phaeodactylumtricornutum | 50%~90%盐度 | 1×106 cells/mL | 35 | 252 | [ |
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