Abstract:

With the rapid development of synthetic biology, traditional labor-intensive research paradigm no longer satisfies the demand from increased numbers for trial-and-error experiments and processing and analysis of mega data. With the support from national, provincial and municipal governments, platform for synthetic biology research, established by Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences is now ready for operation, and expected to put into running in 2023. This report presents a brief introduction to The Major Scientific and Technological Infrastructure for Synthetic Biology Research. The first phase development for the Infrastructure includes three platforms: Design and Learning, Synthesis, and User Testing, and the second phase is for the medical transformation platform. The Infrastructure mainly focus on automated synthetic biotechnology, and introduces the concepts of intelligent manufacturing into synthetic biology research for high-throughput synthesis of living organisms. Through the establishment of such an intelligent production unit based on the information management system, the closed loop of “design-build-test-learn (DBTL)” can be implemented more efficiently with high-throughput and low-cost, which can realize rational and predictable design and synthesis, and also achieve remote design and economical production of synthetic living organisms at large scale. In addition, the platform integrates information technology (IT) and biological technology (BT) through information interconnection and internet of thing (IoT) devices for being fully automated and intelligent to conduct standardized tests, algorithms, processes and other workflows, which ultimately helps to inspire breakthroughs in basic research and interdisciplinary integration for technological innovations.

Key words: synthetic biology, engineering platform, automation platform for synthetic biology, major science and technology infrastructure, design build test learn (DBTL)

摘要：

合成生物学研究中，海量的工程化试错实验远远超出传统的劳动密集型研究范式的能力范畴，故建立一个可以实现生命体工程化大批量合成的合成生物学研究平台迫在眉睫。然而目前国内外已建成的工程化平台只能基于少数孤立设备或功能岛实现部分流程，不能满足合成生物学全生命周期的研究需求。基于此背景，在国家、省市相关部门的大力支持下，由中国科学院深圳先进技术研究院牵头建设的“合成生物研究重大科技基础设施”，目前已完成全部立项程序，进入全面实施建设阶段，预计于2023年开展试运营和验收工作。本文将从建设背景、过程、内容、目标和特色等方面对合成生物研究重大科技基础设施进行介绍。设施工程一期将重点搭建“设计学习”、“合成测试”和“用户检测”三大平台，二期拟建设医学转化平台。合成生物大设施主要围绕自动化合成生物技术，以合成生物学基础研究为理论基础，把自动化工业发展过程中的智能制造、智能工厂理念引入到合成生物学研究中，实现生命体工程化大批量合成。通过建立基于信息管理系统的智能生产单元，快速、低成本、多循环地完成“设计-构建-测试-学习”的闭环，实现理性可预测的设计合成，达成合成生命体的远程定制、异地设计和规模经济生产等目标。同时将信息技术与生物技术交叉融合，发展出适用于自动化、高通量设备平台的标准化实验方法、算法和流程，以期推动合成生物研究过程和工作流程的标准化，进而推动我国合成生物研究水平的提升，成为行业标杆，领跑国际。此外，合成生物大设施还将催动基础研究的原创突破及学科之间的交叉融合，助力生命科学研究实现跨越式发展。

关键词: 合成生物学, 工程化平台, 合成生物学自动化平台, 重大科技基础设施