微藻光驱固碳合成技术的发展现状与未来展望

doi:10.12211/2096-8280.2022-005

合成生物学 ›› 2022, Vol. 3 ›› Issue (5): 884-900.DOI: 10.12211/2096-8280.2022-005

微藻光驱固碳合成技术的发展现状与未来展望

崔金玉¹^,²^,³, 张爱娣¹^,⁴, 栾国栋¹^,²^,³, 吕雪峰¹^,²^,³

^1.中国科学院青岛生物能源与过程研究所，中国科学院生物燃料重点实验室，山东青岛 266101
^2.山东能源研究院，山东青岛 266101
^3.青岛新能源山东省实验室，山东青岛 266101
^4.中南林业科技大学，生命科学与技术学院，湖南长沙 410004

收稿日期:2022-01-19 修回日期:2022-03-31 出版日期:2022-10-31 发布日期:2022-11-16
通讯作者: 吕雪峰
作者简介:崔金玉（1989—），女，博士，博士后。主要从事光合蓝细菌代谢工程相关研究，包括利用合成生物学和系统生物学等策略开发高附加产值化学品和优化底盘菌株抗逆性能。E-mail：cuijinyu@qibebt.ac.cn
吕雪峰（1974—），男，博士，研究员，博士生导师。主要从事合成生物学与绿色生物制造领域的研究，在光驱固碳产能蓝细菌的人工设计与构建及真菌天然产物药物等方面取得系列学术成果。 E-mail：lvxf@qibebt.ac.cn
基金资助:
国家重点研发计划(2021YFA0909700);中国博士后科学基金第70批面上项目(2021M703320)

Engineering microalgae for photosynthetic biosynthesis： progress and prospect

Jinyu CUI¹^,²^,³, Aidi ZHANG¹^,⁴, Guodong LUAN¹^,²^,³, Xuefeng LYU¹^,²^,³

^1.Key Laboratory of Biofuels，Qingdao Institute of Bioenergy and Bioprocess Technology，Chinese Academy of Sciences，Qingdao 266101，Shandong，China
^2.Shandong Energy Institute，Qingdao 266101，Shandong，China
^3.Qingdao New Energy Shandong Laboratory，Qingdao 266101，Shandong，China
^4.College of Life Science and Technology，Central South University of Forestry and Technology，Changsha 410004，Hunan，China

Received:2022-01-19 Revised:2022-03-31 Online:2022-10-31 Published:2022-11-16
Contact: Xuefeng LYU

摘要/Abstract

摘要：

发展CO₂的高效资源化利用技术可同时缓解迫切的环境和能源压力，是实现“双碳”目标的重要途径。微藻是重要的光合固碳微生物，是生物圈初级生产力的主要来源，也是研究光合作用的重要模式体系。近年来，微藻又被视为极具潜力的新型微生物光合平台，具有将太阳能和CO₂直接转化为各种生物基产品的潜力，该生产模式被称为光驱固碳合成技术，可以同时起到固碳减排和绿色合成的效果，是有望助力“双碳”战略目标实现的新型生物制造技术路线。微藻光驱固碳合成技术本质上是通过微藻光合代谢网络重塑实现CO₂的资源化利用，对该方面本文系统总结了“拆盲盒”“挤海绵”“动刀子”3种基本开发模式的研究进展、重要突破和代表性应用示范。而微藻光合代谢网络的深度重塑，又有效扩展了基于微藻光驱固碳合成过程的技术应用场景，在这一方面，本文着重总结微藻生物技术与生物医学、生物光伏、生物航天技术等新型应用场景和技术领域的交叉融合。最后，还针对微藻光驱固碳合成技术在应用中面临的挑战，提出应该重点从合成生物学工具箱开发、高效光合平台开发、规模化培养防污染和防逃逸策略开发等几个环节进行攻关，以加强微藻光驱固碳合成过程的可控制性和可应用性。

关键词: 微藻, 光驱固碳, 细胞工厂, 合成生物学, 开发模式

Abstract:

The development of highly efficient CO₂ utilization technologies can alleviate the urgent pressure on the environment and energy, playing a vital role in achieving the goal of “carbon peak and neutrality”. Microalgae are an important group of photoautotrophic microorganisms, providing the main source of primary productivity in the biosphere, and also serving as important model organisms for photosynthesis research. In recent years, microalgae have also been considered as promising chassis for photosynthetic biosynthesis, directly converting solar energy and carbon dioxide into various bio-based products. This technological route is called photosynthetic biomanufacturing, which possesses the advantages of simultaneous carbon fixation and clean production. This review focuses on the perspectives of development models and application scenarios, and suggests trends related to the further development of photosynthetic biomanufacturing. Regarding the efforts to harness and utilize photosynthetic carbon flow in microalgae cells, we summarized and compared three widely adopted strategies, including novel species screening, environmental perturbations, and genetic engineering. The research progress, significant breakthrough, and representative application demonstration of development models were systematically summarized. In the future, the combination of promising chassis cells with desired industrial properties, systematic metabolic engineering to remodel the native metabolism, and specific environmental treatments to maximize synthesis capacities could be expected to generate next-generation advanced microalgae cell factories. The optimized microalgae cell factories with desired photosynthesis and biosynthesis properties could be expected to play important roles in the areas of biomedical therapy, biophotovoltaics, and bioastronautics through interdisciplinary technology cooperation and integrations. Microalgal synthetic biology is also expect to focus on solving emerging problems rising from new application scenarios and larger application scales, including the development and optimization of the synthetic biology toolboxes, engineering chassis cells toward more efficient photosynthesis, the development of anti-biocontamination and biosafety strategies for large-scale cultivation. Taken together, this review provides useful and updated information to facilitate the development of photosynthetic biosynthesis route with carbon fixation and clean production, providing certain feasible solutions for the "carbon peak and neutrality".

Key words: microalgae, photosynthetic biomanufacturing, cell factory, synthetic biology, development mode

中图分类号:

Q815

崔金玉, 张爱娣, 栾国栋, 吕雪峰. 微藻光驱固碳合成技术的发展现状与未来展望[J]. 合成生物学, 2022, 3(5): 884-900.

Jinyu CUI, Aidi ZHANG, Guodong LUAN, Xuefeng LYU. Engineering microalgae for photosynthetic biosynthesis： progress and prospect[J]. Synthetic Biology Journal, 2022, 3(5): 884-900.

图/表 3

图1 微藻光驱固碳合成技术的开发模式

Fig. 1 The development models of microalgae photosynthetic biomanufacturing

图2 微藻光驱固碳合成过程新颖应用场景[(a) The oxygenic photosynthesis and PDT process using CeCyan cells. These photosensitive CeCyan cells serve as the Ce6 carrier,delivering the photosensitizers to the tumor cells for potential photosynthesis-enhanced PDT under laser irradiation[54]; (b) The engineered strain UTEX 2973, equipped with the photosynthetic machinery and a D-lactate synthesis pathway. The engineered strain S.oneidensis, equipped with a D-lactate oxidation pathway and the extracellular electron transport machinery, releases electrons from D-lactate and transfers them to the anode for electricity production[67]; (c) The bioproduction of a Mars-specific rocket propellant, 2,3-BDO, from CO2, sunlight and water on Mars. Photosynthetic cyanobacteria convert Martian CO2 into sugars that are upgraded by engineered Escherichiacoli into 2,3-BDO[72].] PS I—photosystem I; PS II—photosystem II; ATPase—adenosine triphosphate synthases; CBB cycle—Calvin-Benson-Bassham cycle

Fig. 2 Schematic illustration of new application scenarios of microalgae with desired photosynthesis and biosynthesis properties

图3 开发新应用场景和规模化培养中面临问题的策略PQ—plastoquinone; Cytbf—cytochrome b6f complex; A0—special chlorophyll; A1—vitamin K; 4Fe-4S—iron-sulfur centers; Fd—ferredoxin

Fig. 3 Strategies for solving emerging problems rising from new application scenarios and larger application scales

参考文献 100

1	王永中. 碳达峰、碳中和目标与中国的新能源革命[J]. 人民论坛⋅学术前沿, 2021(14): 88-96.
	WANG Y Z. The targets of carbon peak and carbon neutralization and China's new energy revolution[J]. Frontiers, 2021(14): 88-96.
2	LI Y Q, HORSMAN M, WU N, et al. Biofuels from microalgae[J]. Biotechnology Progress, 2008, 24(4): 815-820.
3	DISMUKES G C, CARRIERI D, BENNETTE N, et al. Aquatic phototrophs: efficient alternatives to land-based crops for biofuels[J]. Current Opinion in Biotechnology, 2008, 19(3): 235-240.
4	GUPTA R S. Molecular signatures for the main phyla of photosynthetic bacteria and their subgroups[J]. Photosynthesis Research, 2010, 104(2/3): 357-372.
5	TORRES-TIJI Y, FIELDS F J, MAYFIELD S P. Microalgae as a future food source[J]. Biotechnology Advances, 2020, 41: 107536.
6	KHAN M, SALMAN M, ANSARI J, et al. Joint external evaluation of IHR core capacities of the Islamic Republic of Pakistan, 2016[J]. International Journal of Infectious Diseases, 2018, 73: 36-37.
7	GOUVEIA L. Microalgae as a feedstock for biofuels[M]// GOUVEIA L. Microalgae as a Feedstock for Biofuels. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011: 1-69.
8	LEVASSEUR W, PERRÉ P, POZZOBON V. A review of high value-added molecules production by microalgae in light of the classification[J]. Biotechnology Advances, 2020, 41: 107545.
9	GROENDAHL S, KAHLERT M, FINK P. The best of both worlds: a combined approach for analyzing microalgal diversity via metabarcoding and morphology-based methods[J]. PLoS One, 2017, 12(2): e0172808.
10	FU W Q, NELSON D R, MYSTIKOU A, et al. Advances in microalgal research and engineering development[J]. Current Opinion in Biotechnology, 2019, 59: 157-164.
11	KUMAR M, SUN Y Q, RATHOUR R, et al. Algae as potential feedstock for the production of biofuels and value-added products: opportunities and challenges[J]. Science of the Total Environment, 2020, 716: 137116.
12	ZHANG J J, WAN L L, XIA S, et al. Morphological and spectrometric analyses of lipids accumulation in a novel oleaginous microalga, Eustigmatos cf. polyphem (Eustigmatophyceae)[J]. Bioprocess and Biosystems Engineering, 2013, 36(8): 1125-1130.
13	GAO B Y, XIA S, LEI X Q, et al. Combined effects of different nitrogen sources and levels and light intensities on growth and fatty acid and lipid production of oleaginous eustigmatophycean microalga Eustigmatos cf. polyphem [J]. Journal of Applied Phycology, 2018, 30(1): 215-229.
14	XU J, LI T, LI C L, et al. Lipid accumulation and eicosapentaenoic acid distribution in response to nitrogen limitation in microalga Eustigmatos vischeri JHsu-01 (Eustigmatophyceae)[J]. Algal Research, 2020, 48: 101910.
15	RAPOSO M F, DE MORAIS R M, BERNARDO DE MORAIS A M. Bioactivity and applications of sulphated polysaccharides from marine microalgae[J]. Marine Drugs, 2013, 11(1): 233-252.
16	GISSIBL A, SUN A, CARE A, et al. Bioproducts from Euglena gracilis: synthesis and applications[J]. Frontiers in Bioengineering and Biotechnology, 2019, 7: 108.
17	SUN A, HASAN M T, HOBBA G, et al. Comparative assessment of the Euglena gracilis var. saccharophila variant strain as a producer of the β-1, 3-glucan paramylon under varying light conditions[J]. Journal of Phycology, 2018, 54(4): 529-538.
18	CHOI S A, JEONG Y, LEE J Y, et al. Biocompatible liquid-type carbon nanodots (C-paints) as light delivery materials for cell growth and astaxanthin induction of Haematococcus pluvialis [J]. Materials Science and Engineering: C, 2020, 109: 110500.
19	WANG F F, GAO B Y, WU M M, et al. A novel strategy for the hyper-production of astaxanthin from the newly isolated microalga Haematococcus pluvialis JNU35[J]. Algal Research, 2019, 39: 101466.
20	YANG Z B, CHENG J, LI K, et al. Optimizing gas transfer to improve growth rate of Haematococcus pluvialis in a raceway pond with chute and oscillating baffles[J]. Bioresource Technology, 2016, 214: 276-283.
21	AMBATI R R, PHANG S M, RAVI S, et al. Astaxanthin: sources, extraction, stability, biological activities and its commercial applications-a review[J]. Marine Drugs, 2014, 12(1): 128-152.
22	YANG J S, CAO J, XING G L, et al. Lipid production combined with biosorption and bioaccumulation of cadmium, copper, manganese and zinc by oleaginous microalgae Chlorella minutissima UTEX2341[J]. Bioresource Technology, 2015, 175: 537-544.
23	CHEN J H, WEI D, LIM P E. Enhanced coproduction of astaxanthin and lipids by the green microalga Chromochloris zofingiensis: selected phytohormones as positive stimulators[J]. Bioresource Technology, 2020, 295: 122242.
24	HAGEMANN M. Molecular biology of cyanobacterial salt acclimation[J]. FEMS Microbiology Reviews, 2011, 35(1): 87-123.
25	QIAO Y, WANG W H, LU X F. Engineering cyanobacteria as cell factories for direct trehalose production from CO₂ [J]. Metabolic Engineering, 2020, 62: 161-171.
26	CUI J Y, SUN T, CHEN L, et al. Salt-tolerant Synechococcus elongatus UTEX 2973 obtained via engineering of heterologous synthesis of compatible solute glucosylglycerol[J]. Frontiers in Microbiology, 2021, 12: 650217.
27	TAN X M, DU W, LU X F. Photosynthetic and extracellular production of glucosylglycerol by genetically engineered and gel-encapsulated cyanobacteria[J]. Applied Microbiology and Biotechnology, 2015, 99(5): 2147-2154.
28	LEVER M, SLOW S. The clinical significance of betaine, an osmolyte with a key role in methyl group metabolism[J]. Clinical Biochemistry, 2010, 43(9): 732-744.
29	DAY C R, KEMPSON S A. Betaine chemistry, roles, and potential use in liver disease[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2016, 1860(6): 1098-1106.
30	RAY A, NAYAK M, GHOSH A. A review on co-culturing of microalgae: a greener strategy towards sustainable biofuels production[J]. Science of the Total Environment, 2022, 802: 149765.
31	XU L L, LI D Z, WANG Q X, et al. Improved hydrogen production and biomass through the co-cultivation of Chlamydomonas reinhardtii and Bradyrhizobium japonicum [J]. International Journal of Hydrogen Energy, 2016, 41(22): 9276-9283.
32	XU L L, CHENG X L, WU S X, et al. Co-cultivation of Chlamydomonas reinhardtii with Azotobacter chroococcum improved H₂ production[J]. Biotechnology Letters, 2017, 39(5): 731-738.
33	OUYANG Y, CHEN S Y, ZHAO L Q, et al. Global metabolomics reveals that Vibrio natriegens enhances the growth and paramylon synthesis of Euglena gracilis [J]. Frontiers in Bioengineering and Biotechnology, 2021, 9: 652021.
34	张丽, 宋馨宇, 陈磊, 等. 光合微生物混菌体系的应用和研究进展[J]. 生物工程学报, 2020, 36(4): 652-665.
	ZHANG L, SONG X Y, CHEN L, et al. Recent progress in photosynthetic microbial co-culture systems[J]. Chinese Journal of Biotechnology, 2020, 36(4): 652-665.
35	LI T T, LI C T, BUTLER K, et al. Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform[J]. Biotechnology for Biofuels, 2017, 10: 55.
36	STANIER R Y, COHEN-BAZIRE G. Phototrophic prokaryotes: the cyanobacteria[J]. Annual Review of Microbiology, 1977, 31: 225-274.
37	SUN T, LI S B, SONG X Y, et al. Toolboxes for cyanobacteria: recent advances and future direction[J]. Biotechnology Advances, 2018, 36(4): 1293-1307.
38	SANTOS-MERINO M, SINGH A K, DUCAT D C. New applications of synthetic biology tools for cyanobacterial metabolic engineering[J]. Frontiers in Bioengineering and Biotechnology, 2019, 7: 33.
39	GAO X Y, SUN T, PEI G S, et al. Cyanobacterial chassis engineering for enhancing production of biofuels and chemicals[J]. Applied Microbiology and Biotechnology, 2016, 100(8): 3401-3413.
40	DENG M D, COLEMAN J R. Ethanol synthesis by genetic engineering in cyanobacteria[J]. Applied and Environmental Microbiology, 1999, 65(2): 523-528.
41	GAO Z X, ZHAO H, LI Z M, et al. Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria[J]. Energy & Environmental Science, 2012, 5(12): 9857-9865.
42	GAO X, GAO F, LIU D, et al. Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO₂ [J]. Energy & Environmental Science, 2016, 9(4): 1400-1411.
43	DUNAHAY T G, JARVIS E E, DAIS S S, et al. Manipulation of microalgal lipid production using genetic engineering[J]. Applied Biochemistry and Biotechnology, 1996, 57/58(1): 223-231.
44	HAMILTON M L, HASLAM R P, NAPIER J A, et al. Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of ω-3 long chain polyunsaturated fatty acids[J]. Metabolic Engineering, 2014, 22: 3-9.
45	XIN Y, LU Y D, LEE Y Y, et al. Producing designer oils in industrial microalgae by rational modulation of co-evolving type-2 diacylglycerol acyltransferases[J]. Molecular Plant, 2017, 10(12): 1523-1539.
46	JEON S, KOH H G, CHO J M, et al. Enhancement of lipid production in Nannochloropsis salina by overexpression of endogenous NADP-dependent malic enzyme[J]. Algal Research, 2021, 54: 102218.
47	DAVIES F K, WORK V H, BELIAEV A S, et al. Engineering limonene and bisabolene production in wild type and a glycogen-deficient mutant of Synechococcus sp. PCC 7002[J]. Frontiers in Bioengineering and Biotechnology, 2014, 2: 21.
48	LIU Y M, CUI Y L, CHEN J, et al. Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin[J]. Algal Research, 2019, 44: 101679.
49	MUZ B, DE LA PUENTE P, AZAB F, et al. The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy[J]. Hypoxia, 2015, 3: 83-92.
50	DOLMANS D E J G J, FUKUMURA D, JAIN R K. Photodynamic therapy for cancer[J]. Nature Reviews Cancer, 2003, 3(5): 380-387.
51	HU T T, WANG Z D, SHEN W C, et al. Recent advances in innovative strategies for enhanced cancer photodynamic therapy[J]. Theranostics, 2021, 11(7): 3278-3300.
52	CHEN Q, FENG L Z, LIU J J, et al. Intelligent albumin-MnO₂ nanoparticles as pH-/ H₂O₂-responsive dissociable nanocarriers to modulate tumor hypoxia for effective combination therapy[J]. Advanced Materials, 2016, 28(33): 7129-7136.
53	LIU L L, HE H M, LUO Z Y, et al. In situ photocatalyzed oxygen generation with photosynthetic bacteria to enable robust immunogenic photodynamic therapy in triple-negative breast cancer[J]. Advanced Functional Materials, 2020, 30(10): 1910176.
54	HUO M F, WANG L Y, ZHANG L L, et al. Photosynthetic tumor oxygenation by photosensitizer-containing cyanobacteria for enhanced photodynamic therapy[J]. Angewandte Chemie (International Ed in English), 2020, 59(5): 1906-1913.
55	SUN T, ZHANG Y Y, ZHANG C N, et al. Cyanobacteria-based bio-oxygen pump promoting hypoxia-resistant photodynamic therapy[J]. Frontiers in Bioengineering and Biotechnology, 2020, 8: 237.
56	ZHANG Y H, LIU H F, DAI X Y, et al. Cyanobacteria-based near-infrared light-excited self-supplying oxygen system for enhanced photodynamic therapy of hypoxic tumors[J]. Nano Research, 2021, 14(3): 667-673.
57	GUO M M, WANG S C, GUO Q L, et al. NIR-responsive spatiotemporally controlled cyanobacteria micro-nanodevice for intensity-modulated chemotherapeutics in rheumatoid arthritis[J]. ACS Applied Materials & Interfaces, 2021, 13(16): 18423-18431.
58	LEE C, LIM K, KIM S S, et al. Chlorella-gold nanorods hydrogels generating photosynthesis-derived oxygen and mild heat for the treatment of hypoxic breast cancer[J]. Journal of Controlled Release, 2019, 294: 77-90.
59	孙晨凯, 陈鑫, 程皓, 等. 增氧型纳米递送系统用于光动力治疗的研究进展[J]. 中国药科大学学报, 2021, 52(4): 387-397.
	SUN C K, CHEN X, CHENG H, et al. Advances of research on oxygen-enhancing nano-delivery system for photodynamic therapy[J]. Journal of China Pharmaceutical University, 2021, 52(4): 387-397.
60	HU H, QIAN X Q, CHEN Y. Microalgae-enabled photosynthetic alleviation of tumor hypoxia for enhanced nanotherapies[J]. Science Bulletin, 2020, 65(22): 1869-1871.
61	WANG H R, GUO Y F, WANG C, et al. Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia[J]. Biomaterials, 2021, 269: 120621.
62	COHEN J E, GOLDSTONE A B, PAULSEN M J, et al. An innovative biologic system for photon-powered myocardium in the ischemic heart[J]. Science Advances, 2017, 3(6): e1603078.
63	ÖZUGUR S, CHÁVEZ M N, SANCHEZ-GONZALEZ R, et al. Green oxygen power plants in the brain rescue neuronal activity[J]. iScience, 2021, 24(10): 103158.
64	POLMAN A, KNIGHT M, GARNETT E C, et al. Photovoltaic materials: present efficiencies and future challenges[J]. Science, 2016, 352(6283): aad4424.
65	GREENMAN J, GAJDA I, IEROPOULOS I. Microbial fuel cells (MFC) and microalgae; photo microbial fuel cell (PMFC) as complete recycling machines[J]. Sustainable Energy & Fuels, 2019, 3(10): 2546-2560.
66	MCCORMICK A J, BOMBELLI P, BRADLEY R W, et al. Biophotovoltaics: oxygenic photosynthetic organisms in the world of bioelectrochemical systems[J]. Energy & Environmental Science, 2015, 8(4): 1092-1109.
67	LEA-SMITH D J, BOMBELLI P, VASUDEVAN R, et al. Photosynthetic, respiratory and extracellular electron transport pathways in cyanobacteria[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2016, 1857(3): 247-255.
68	ZHU H W, MENG H K, ZHANG W, et al. Development of a longevous two-species biophotovoltaics with constrained electron flow[J]. Nature Communications, 2019, 10: 4282.
69	FIROOZABADI H, MARDANPOUR M M, MOTAMEDIAN E. A system-oriented strategy to enhance electron production of Synechocystis sp. PCC6803 in bio-photovoltaic devices: experimental and modeling insights[J]. Scientific Reports, 2021, 11: 12294.
70	赵贞尧, 张保财, 李锋, 等. 产电细胞的合成生物学设计构建[J]. 化工学报, 2021, 72(1): 468-482.
	ZHAO Z Y, ZHANG B C, LI F, et al. Design and construction of exoelectrogens by synthetic biology[J]. CIESC Journal, 2021, 72(1): 468-482.
71	SEKAR N, JAIN R, YAN Y J, et al. Enhanced photo-bioelectrochemical energy conversion by genetically engineered cyanobacteria[J]. Biotechnology and Bioengineering, 2016, 113(3): 675-679.
72	KRUYER N S, REALFF M J, SUN W T, et al. Designing the bioproduction of Martian rocket propellant via a biotechnology-enabled in situ resource utilization strategy[J]. Nature Communications, 2021, 12: 6166.
73	WENDT K E, UNGERER J, COBB R E, et al. CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973[J]. Microbial Cell Factories, 2016, 15(1): 115.
74	UNGERER J, PAKRASI H B. Cpf1 is a versatile tool for CRISPR genome editing across diverse species of cyanobacteria[J]. Scientific Reports, 2016, 6: 39681.
75	LIU D, JOHNSON V M, PAKRASI H B. A reversibly induced CRISPRi system targeting photosystem II in the cyanobacterium Synechocystis sp. PCC 6803[J]. ACS Synthetic Biology, 2020, 9(6): 1441-1449.
76	CHOI S Y, WOO H M. CRISPRi-dCas12a: a dCas12a-mediated CRISPR interference for repression of multiple genes and metabolic engineering in cyanobacteria[J]. ACS Synthetic Biology, 2020, 9(9): 2351-2361.
77	BADIS Y, SCORNET D, HARADA M, et al. Targeted CRISPR-Cas9-based gene knockouts in the model brown alga Ectocarpus [J]. The New Phytologist, 2021, 231(5): 2077-2091.
78	ZHANG M Y, LUO Q, SUN H L, et al. Engineering a controllable targeted protein degradation system and a derived OR-GATE-type inducible gene expression system in Synechococcus elongatus PCC 7942[J]. ACS Synthetic Biology, 2022, 11(1): 125-134.
79	CUI J Y, SUN T, CHEN L, et al. Engineering salt tolerance of photosynthetic cyanobacteria for seawater utilization[J]. Biotechnology Advances, 2020, 43: 107578.
80	JAISWAL D, SENGUPTA A, SOHONI S, et al. Genome features and biochemical characteristics of a robust, fast growing and naturally transformable cyanobacterium Synechococcus elongatus PCC 11801 isolated from India[J]. Scientific Reports, 2018, 8: 16632.
81	PRASANNAN C B, JAISWAL D, DAVIS R, et al. An improved method for extraction of polar and charged metabolites from cyanobacteria[J]. PLoS One, 2018, 13(10): e0204273.
82	WŁODARCZYK A, SELÃO T T, NORLING B, et al. Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production[J]. Communications Biology, 2020, 3: 215.
83	DE ALVARENGA L V, LUCIUS S, VAZ M G M V, et al. The novel strain Desmonostoc salinum CCM-UFV059 shows higher salt and desiccation resistance compared to the model strain Nostoc sp. PCC7120[J]. Journal of Phycology, 2020, 56(2): 496-506.
84	DU W, BURBANO P C, HELLINGWERF K J, et al. Challenges in the application of synthetic biology toward synthesis of commodity products by cyanobacteria via “direct conversion”[J]. Advances in Experimental Medicine and Biology, 2018, 1080: 3-26.
85	LUAN G D, LU X F. Tailoring cyanobacterial cell factory for improved industrial properties[J]. Biotechnology Advances, 2018, 36(2): 430-442.
86	LUAN G D, ZHANG S S, LU X F. Engineering cyanobacteria chassis cells toward more efficient photosynthesis[J]. Current Opinion in Biotechnology, 2020, 62: 1-6.
87	PATHANIA R, SRIVASTAVA A, SRIVASTAVA S, et al. Metabolic systems biology and multi-omics of cyanobacteria: perspectives and future directions[J]. Bioresource Technology, 2022, 343: 126007.
88	LI Z D, WU C, GAO X, et al. Exogenous electricity flowing through cyanobacterial photosystem I drives CO₂ valorization with high energy efficiency[J]. Energy & Environmental Science, 2021, 14(10): 5480-5490.
89	MARY LEEMA J T, KIRUBAGARAN R, VINITHKUMAR N V, et al. High value pigment production from Arthrospira (Spirulina) platensis cultured in seawater[J]. Bioresource Technology, 2010, 101(23): 9221-9227.
90	TOULOUPAKIS E, CICCHI B, BENAVIDES A M S, et al. Effect of high pH on growth of Synechocystis sp. PCC 6803 cultures and their contamination by golden algae (Poterioochromonas sp.)[J]. Applied Microbiology and Biotechnology, 2016, 100(3): 1333-1341.
91	ZHU Z, LUAN G D, TAN X M, et al. Rescuing ethanol photosynthetic production of cyanobacteria in non-sterilized outdoor cultivations with a bicarbonate-based pH-rising strategy[J]. Biotechnology for Biofuels, 2017, 10: 93.
92	SHAW A J, LAM F H, HAMILTON M, et al. Metabolic engineering of microbial competitive advantage for industrial fermentation processes[J]. Science, 2016, 353(6299): 583-586.
93	SELÃO T T, WŁODARCZYK A, NIXON P J, et al. Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sources[J]. Metabolic Engineering, 2019, 54: 255-263.
94	LÉVESQUE B, GERVAIS M C, CHEVALIER P, et al. Prospective study of acute health effects in relation to exposure to cyanobacteria[J]. Science of the Total Environment, 2014, 466/467: 397-403.
95	PORTER R D. Transformation in cyanobacteria[J]. CRC Critical Reviews in Microbiology, 1986, 13(2): 111-132.
96	JIA B, QI H, LI B Z, et al. Orthogonal ribosome biofirewall[J]. ACS Synthetic Biology, 2017, 6(11): 2108-2117.
97	LOERA-QUEZADA M M, LEYVA-GONZÁLEZ M A, VELÁZQUEZ-JUÁREZ G, et al. A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae[J]. Plant Biotechnology Journal, 2016, 14(10): 2066-2076.
98	MOTOMURA K, SANO K, WATANABE S, et al. Synthetic phosphorus metabolic pathway for biosafety and contamination management of cyanobacterial cultivation[J]. ACS Synthetic Biology, 2018, 7(9): 2189-2198.
99	ZHOU Y Q, SUN T, CHEN Z X, et al. Development of a new biocontainment strategy in model cyanobacterium Synechococcus strains[J]. ACS Synthetic Biology, 2019, 8(11): 2576-2584.
100	孟凡康, 娄春波. 人工遗传改造生命体的防逃逸技术研究进展[J]. 有机化学, 2018, 38(9): 2231-2242.
	MENG F K, LOU C B. Research progress in biocontainment of genetically modified organisms[J]. Chinese Journal of Organic Chemistry, 2018, 38(9): 2231-2242.

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微藻光驱固碳合成技术的发展现状与未来展望

Engineering microalgae for photosynthetic biosynthesis： progress and prospect

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