Synthetic Biology Journal ›› 2022, Vol. 3 ›› Issue (2): 399-414.DOI: 10.12211/2096-8280.2021-048
• Invited Review • Previous Articles Next Articles
Xinxin XU1,2, Hua KUANG1,2
Received:
2021-04-21
Revised:
2021-09-13
Online:
2022-05-11
Published:
2022-04-30
Contact:
Hua KUANG
胥欣欣1,2, 匡华1,2
通讯作者:
匡华
作者简介:
基金资助:
CLC Number:
Xinxin XU, Hua KUANG. Advances in the biological detection of food contaminants based on synthetic receptors[J]. Synthetic Biology Journal, 2022, 3(2): 399-414.
胥欣欣, 匡华. 基于合成受体的食品污染物生物检测进展[J]. 合成生物学, 2022, 3(2): 399-414.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2021-048
表达系统 | 优势 | 劣势 |
---|---|---|
原核表达系统 | 操作简单,培养周期短,成本低廉,表达量高 | 无翻译后修饰,易形成包含体 |
酵母表达系统 | 遗传背景清楚,操作简单,培养周期短,成本低廉,表达量高,无内毒素污染,简单的翻译后修饰 | 糖基化修饰与天然蛋白有差异 |
哺乳动物表达系统 | 糖基化修饰完整,蛋白折叠正确、活性高,最接近天然蛋白 | 周期长,产率低,耗费高 |
昆虫表达系统 | 翻译后修饰系统比原核和酵母系统更加完善,生产周期比哺乳动物系统短,重组蛋白产量高 | 病毒侵染导致细胞死亡,可能使得蛋白修饰不完整;糖基化修饰与天然蛋白有差异 |
Tab. 1 Comparison of 4 expression systems
表达系统 | 优势 | 劣势 |
---|---|---|
原核表达系统 | 操作简单,培养周期短,成本低廉,表达量高 | 无翻译后修饰,易形成包含体 |
酵母表达系统 | 遗传背景清楚,操作简单,培养周期短,成本低廉,表达量高,无内毒素污染,简单的翻译后修饰 | 糖基化修饰与天然蛋白有差异 |
哺乳动物表达系统 | 糖基化修饰完整,蛋白折叠正确、活性高,最接近天然蛋白 | 周期长,产率低,耗费高 |
昆虫表达系统 | 翻译后修饰系统比原核和酵母系统更加完善,生产周期比哺乳动物系统短,重组蛋白产量高 | 病毒侵染导致细胞死亡,可能使得蛋白修饰不完整;糖基化修饰与天然蛋白有差异 |
抗生素类型 | 受体蛋白 | 检测方式 | 检测基质 | 可检测种类数 | LOD/(ng/mL) | IC50/(ng/mL) | 参考文献 |
---|---|---|---|---|---|---|---|
β-内酰胺类 | PBP2x* | ELISA | 牛奶 | 15 | 0.08~27.45 | — | [ |
sPBP3* | ELISA | PBS/牛奶 | 27/13 | 0.26~109.46/0.52~27.40 | 2.13~426.02 | [ | |
PBP2x* | ELISA | 牛奶、肉汁、鸡蛋、蜂蜜 | 6 | — | — | [ | |
BlaR-CTD | ELISA | 牛奶、牛肉、鸡肉 | 11 | — | 0.18~170.81 | [ | |
BlaR-CTD | ELISA | 13种食物 | 40 | — | — | [ | |
BlaR-CTD | GICA | 牛奶、鸡肉 | 21 | 低于限量要求 | — | [ | |
PBP-6 | SERS-LFIA | 牛奶 | 1 | 0.01 | 1.77 | [ | |
磺胺类 | DHPS | ELISA | 牛奶 | 28 | — | 426~50 000 | [ |
DHPS-DHPPP | FPA/ELISA | 牛奶 | 29 | 1.6~59/ 1.15~14.91 | <100 | [ | |
DHPS-DHPPP | ELISA | 鸡肉、猪肉、鸡蛋、蜂蜜 | 1 | 5.57~23.22 | — | [ | |
四环素类 | TetR-tetO | ELISA | 牛奶、牛血清 | 8 | 0.1~7.2 | — | [ |
TetR-tetO | 试纸条法 | 牛奶、肉末、牛血清 | 8 | — | — | [ | |
TetR | CL-ELISA | 牛奶 | 5 | 0.005~0.016 | 0.5~2.2 | [ | |
TetR | ELISA | 鸡蛋 | 9 | 0.3~5.8 | 3.1~17.2 | [ | |
TetR-tetO | 化学发光微流控条 | 自来水 | 1 | 0.1 | — | [ | |
大环内酯类 | MphR(A) | 细胞传感器 | — | 1 | — | — | [ |
MphR(A) | 微生物传感器 | — | 1 | — | — | [ | |
MphR(A) | ELISA | 牛奶、牛血清 | 5 | 1.7~5000 | — | [ | |
MphR(A)、MphR(E) | ELISA | 原奶 | 1 | — | — | [ |
Tab. 2 Receptor-based antibiotic screening and analysis method
抗生素类型 | 受体蛋白 | 检测方式 | 检测基质 | 可检测种类数 | LOD/(ng/mL) | IC50/(ng/mL) | 参考文献 |
---|---|---|---|---|---|---|---|
β-内酰胺类 | PBP2x* | ELISA | 牛奶 | 15 | 0.08~27.45 | — | [ |
sPBP3* | ELISA | PBS/牛奶 | 27/13 | 0.26~109.46/0.52~27.40 | 2.13~426.02 | [ | |
PBP2x* | ELISA | 牛奶、肉汁、鸡蛋、蜂蜜 | 6 | — | — | [ | |
BlaR-CTD | ELISA | 牛奶、牛肉、鸡肉 | 11 | — | 0.18~170.81 | [ | |
BlaR-CTD | ELISA | 13种食物 | 40 | — | — | [ | |
BlaR-CTD | GICA | 牛奶、鸡肉 | 21 | 低于限量要求 | — | [ | |
PBP-6 | SERS-LFIA | 牛奶 | 1 | 0.01 | 1.77 | [ | |
磺胺类 | DHPS | ELISA | 牛奶 | 28 | — | 426~50 000 | [ |
DHPS-DHPPP | FPA/ELISA | 牛奶 | 29 | 1.6~59/ 1.15~14.91 | <100 | [ | |
DHPS-DHPPP | ELISA | 鸡肉、猪肉、鸡蛋、蜂蜜 | 1 | 5.57~23.22 | — | [ | |
四环素类 | TetR-tetO | ELISA | 牛奶、牛血清 | 8 | 0.1~7.2 | — | [ |
TetR-tetO | 试纸条法 | 牛奶、肉末、牛血清 | 8 | — | — | [ | |
TetR | CL-ELISA | 牛奶 | 5 | 0.005~0.016 | 0.5~2.2 | [ | |
TetR | ELISA | 鸡蛋 | 9 | 0.3~5.8 | 3.1~17.2 | [ | |
TetR-tetO | 化学发光微流控条 | 自来水 | 1 | 0.1 | — | [ | |
大环内酯类 | MphR(A) | 细胞传感器 | — | 1 | — | — | [ |
MphR(A) | 微生物传感器 | — | 1 | — | — | [ | |
MphR(A) | ELISA | 牛奶、牛血清 | 5 | 1.7~5000 | — | [ | |
MphR(A)、MphR(E) | ELISA | 原奶 | 1 | — | — | [ |
非法添加剂类型 | 受体蛋白 | 检测方式 | 检测基质 | LOD/(ng/mL) | IC50/(ng/mL) | 参考文献 |
---|---|---|---|---|---|---|
β兴奋剂 | β兴奋剂受体 | ELISA | 药片 | — | — | [ |
β2-AR | ELRA | 猪尿 | — | 34~63 | [ | |
β2-AR | ELRA | 猪尿 | — | 45.99~78.02 | [ | |
β2-AR | ELRA | 猪尿 | — | 28.36~59.57 | [ | |
β2-AR | ELRA | — | 5.20 | 30.38 | [ | |
PDE5抑制剂 | 磷酸二酯酶PDE5 | 荧光检测方法 | 膳食补充剂 | — | 0.4~4.0 | [ |
Tab. 3 Receptor-based screening and analysis method of illegal additives
非法添加剂类型 | 受体蛋白 | 检测方式 | 检测基质 | LOD/(ng/mL) | IC50/(ng/mL) | 参考文献 |
---|---|---|---|---|---|---|
β兴奋剂 | β兴奋剂受体 | ELISA | 药片 | — | — | [ |
β2-AR | ELRA | 猪尿 | — | 34~63 | [ | |
β2-AR | ELRA | 猪尿 | — | 45.99~78.02 | [ | |
β2-AR | ELRA | 猪尿 | — | 28.36~59.57 | [ | |
β2-AR | ELRA | — | 5.20 | 30.38 | [ | |
PDE5抑制剂 | 磷酸二酯酶PDE5 | 荧光检测方法 | 膳食补充剂 | — | 0.4~4.0 | [ |
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