Synthetic Biology Journal ›› 2023, Vol. 4 ›› Issue (6): 1300-1320.DOI: 10.12211/2096-8280.2022-052
• Research Article • Previous Articles
Qingxiang ZHENG
Received:
2022-09-23
Revised:
2022-11-16
Online:
2024-01-19
Published:
2023-12-31
Contact:
Qingxiang ZHENG
郑庆祥
通讯作者:
郑庆祥
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Qingxiang ZHENG. Metabolic flux analysis of Shewanella sp. MR-4 with different terminal electron receptors[J]. Synthetic Biology Journal, 2023, 4(6): 1300-1320.
郑庆祥. 希瓦氏菌Shewanella sp. MR-4在不同末端电子受体条件下的代谢通量分析[J]. 合成生物学, 2023, 4(6): 1300-1320.
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URL: https://synbioj.cip.com.cn/EN/10.12211/2096-8280.2022-052
碱性溶液 Basic solution | ||
---|---|---|
成分 Component | 浓度/(mmol/L) Concentration/(mmol/L) | |
PIPES | 30 | |
NH4Cl | 28.04 | |
KCl | 1.34 | |
NaH2PO4·2H2O | 4.35 | |
NaCl | 30 | |
Na2SO4 | 0.7 | |
矿物溶液 Mineral solution | ||
成分 Component | 浓度/(µmol/L) Concentration/ (µmol/L) | |
C6H9NO6 | 78.49 | |
MgSO4·7H2O | 121.71 | |
MnSO4·H2O | 29.58 | |
FeSO4·7H2O | 3.6 | |
CaCl2·2H2O | 6.8 | |
CoCl2·6H2O | 4.2 | |
ZnCl2 | 9.54 | |
CuSO4·5H2O | 0.4 | |
AlK(SO4)2·12H2O | 0.21 | |
H3BO3 | 1.62 | |
Na2MoO4·2H2O | 1.03 | |
NiCl2·6H2O | 1.01 | |
Na2WO4·2H2O | 0.76 | |
维生素溶液 Vitamin solution | ||
成分 Component | 浓度/(µmol/L) Concentration/(µmol/L) | |
D-生物素 | D-biotin | 81.87 |
叶酸 | folic acid | 45.34 |
盐酸吡哆醇 | pyridoxine HCl | 486.38 |
核黄素 | riboflavin | 132.84 |
盐酸硫胺素 | thiamine HCl | 140.73 |
烟碱酸 | nicotinic acid | 406.17 |
D-泛酸,半钙盐 | D-pantothenic acid, hemi calcium salt | 209.82 |
维生素B12 | VB12 | 7.4 |
对氨基苯甲酸 | p-amino benzoic acid | 364.62 |
硫辛酸 | thioctic acid | 242.37 |
氯化胆碱 | choline chloride | 14.3246 |
Table 1 Modified M1 minimal medium
碱性溶液 Basic solution | ||
---|---|---|
成分 Component | 浓度/(mmol/L) Concentration/(mmol/L) | |
PIPES | 30 | |
NH4Cl | 28.04 | |
KCl | 1.34 | |
NaH2PO4·2H2O | 4.35 | |
NaCl | 30 | |
Na2SO4 | 0.7 | |
矿物溶液 Mineral solution | ||
成分 Component | 浓度/(µmol/L) Concentration/ (µmol/L) | |
C6H9NO6 | 78.49 | |
MgSO4·7H2O | 121.71 | |
MnSO4·H2O | 29.58 | |
FeSO4·7H2O | 3.6 | |
CaCl2·2H2O | 6.8 | |
CoCl2·6H2O | 4.2 | |
ZnCl2 | 9.54 | |
CuSO4·5H2O | 0.4 | |
AlK(SO4)2·12H2O | 0.21 | |
H3BO3 | 1.62 | |
Na2MoO4·2H2O | 1.03 | |
NiCl2·6H2O | 1.01 | |
Na2WO4·2H2O | 0.76 | |
维生素溶液 Vitamin solution | ||
成分 Component | 浓度/(µmol/L) Concentration/(µmol/L) | |
D-生物素 | D-biotin | 81.87 |
叶酸 | folic acid | 45.34 |
盐酸吡哆醇 | pyridoxine HCl | 486.38 |
核黄素 | riboflavin | 132.84 |
盐酸硫胺素 | thiamine HCl | 140.73 |
烟碱酸 | nicotinic acid | 406.17 |
D-泛酸,半钙盐 | D-pantothenic acid, hemi calcium salt | 209.82 |
维生素B12 | VB12 | 7.4 |
对氨基苯甲酸 | p-amino benzoic acid | 364.62 |
硫辛酸 | thioctic acid | 242.37 |
氯化胆碱 | choline chloride | 14.3246 |
菌种 | 乳酸盐(lactate) 氧气(oxygen) 有氧(aerobic) | 乳酸盐(lactate) 延胡索酸(fumarate) 厌氧(anaerobic) | 葡萄糖(glucose) 延胡索酸(fumarate) 厌氧(anaerobic) | 葡萄糖(glucose) 次氮基三乙酸铁(Fe-NTA) 厌氧(anaerobic) |
---|---|---|---|---|
Shewanella putrefaciens CN-32 | + | + | + | - |
Shewanella loihica PV-4 | + | + | + | + |
Shewanella sp. MR-4 | + | + | + | + |
Shewanella amazonensis SB2B | + | + | + | + |
Shewanella sp. W3-18-1 | + | + | + | - |
Shewanella baltica OS155 | + | + | + | + |
Shewanella denitrificans OS217 | + | + | - | - |
Shewanella oneidensis MR-1 | + | + | - | - |
Shewanella sp. MR-7 | + | + | - | + |
Shewanella sp. ANA-3 | + | + | + | + |
Shewanella pealeana ANG-SQ1 | + | - | - | - |
Shewanella frigidimarina NCIMB400 | + | + | - | - |
Table 2 Growth phenotype of 12 Shewanella sp. strains
菌种 | 乳酸盐(lactate) 氧气(oxygen) 有氧(aerobic) | 乳酸盐(lactate) 延胡索酸(fumarate) 厌氧(anaerobic) | 葡萄糖(glucose) 延胡索酸(fumarate) 厌氧(anaerobic) | 葡萄糖(glucose) 次氮基三乙酸铁(Fe-NTA) 厌氧(anaerobic) |
---|---|---|---|---|
Shewanella putrefaciens CN-32 | + | + | + | - |
Shewanella loihica PV-4 | + | + | + | + |
Shewanella sp. MR-4 | + | + | + | + |
Shewanella amazonensis SB2B | + | + | + | + |
Shewanella sp. W3-18-1 | + | + | + | - |
Shewanella baltica OS155 | + | + | + | + |
Shewanella denitrificans OS217 | + | + | - | - |
Shewanella oneidensis MR-1 | + | + | - | - |
Shewanella sp. MR-7 | + | + | - | + |
Shewanella sp. ANA-3 | + | + | + | + |
Shewanella pealeana ANG-SQ1 | + | - | - | - |
Shewanella frigidimarina NCIMB400 | + | + | - | - |
电子受体 (Electron acceptor) | 用于生物质合成的 葡萄糖的比例 (Percentage of glucose for biomass synthesis) | 比率/[mmol/(gCDW·h)] Specific rate/[mmol/(gCDW·h)] | 碳平衡 (Carbon balance) | |||
---|---|---|---|---|---|---|
葡萄糖摄取 (Glucose uptake) | 醋酸盐分泌 (Acetate secretion) | EPS形成 (EPS formation) | CO2净生成 (Net CO2 formation) | |||
硝酸盐(Nitrate) | 12.2% ± 0.5% | 8.27 ± 0.24 | 4.19 ± 0.21 | 2.49 ± 0.11 | 17.47 ± 0.94 | 94.5% |
Fe | 5.1% ± 0.2% | 4.86 ± 0.20 | 3.74 ± 0.19 | 1.28 ± 0.04 | 10.72 ± 0.35 | 93.9% |
延胡索酸(Fumarate) | 11.7%± 0.4% | 5.04 ± 0.17 | 8.33 ± 0.54 | 0.31 ± 0.01 | 7.23 ± 0.38 | 96.8% |
DMSO | 5.9% ± 0.2% | 5.38 ± 0.15 | 9.48 ± 0.47 | 0.47 ± 0.02 | 6.92 ± 0.21 | 94.9% |
Table 3 Anaerobic growth parameters of Shewanella sp. MR-4 exposed to various terminal electron acceptors
电子受体 (Electron acceptor) | 用于生物质合成的 葡萄糖的比例 (Percentage of glucose for biomass synthesis) | 比率/[mmol/(gCDW·h)] Specific rate/[mmol/(gCDW·h)] | 碳平衡 (Carbon balance) | |||
---|---|---|---|---|---|---|
葡萄糖摄取 (Glucose uptake) | 醋酸盐分泌 (Acetate secretion) | EPS形成 (EPS formation) | CO2净生成 (Net CO2 formation) | |||
硝酸盐(Nitrate) | 12.2% ± 0.5% | 8.27 ± 0.24 | 4.19 ± 0.21 | 2.49 ± 0.11 | 17.47 ± 0.94 | 94.5% |
Fe | 5.1% ± 0.2% | 4.86 ± 0.20 | 3.74 ± 0.19 | 1.28 ± 0.04 | 10.72 ± 0.35 | 93.9% |
延胡索酸(Fumarate) | 11.7%± 0.4% | 5.04 ± 0.17 | 8.33 ± 0.54 | 0.31 ± 0.01 | 7.23 ± 0.38 | 96.8% |
DMSO | 5.9% ± 0.2% | 5.38 ± 0.15 | 9.48 ± 0.47 | 0.47 ± 0.02 | 6.92 ± 0.21 | 94.9% |
反应编号 (Reaction No.) | 酶 (Enzyme) | 基因 (Gene) | 反应化学计量学 (Stoichiometric reaction) | 碳原子转换 (Carbon atom transition) |
---|---|---|---|---|
v1 | glucokinase | Shewmr4_1763 | Glucose => G6P | #ABCDEF => #ABCDEF |
v2 | glucose-6-phosphate 1-dehydrogenase | Shewmr4_2046 | G6P => 6PG | #ABCDEF => #ABCDEF |
6-phosphogluconolactonase | Shewmr4_2045 | |||
v3 | phosphogluconate dehydratase | Shewmr4_2044 | 6PG => KDPG | #ABCDEF => #ABCDEF |
2-keto-3-deoxy-phosphogluconate aldolase | Shewmr4_2043 | KDPG => GAP + PYR | #ABCDEF => #DEF + #ABC | |
v4 | glucose-6-phosphate isomerase | Shewmr4_2968 | G6P <=> F6P | #ABCDEF <=> #ABCDEF |
v5 | 6-phosphogluconate dehydrogenase | Shewmr4_1609 | 6PG => P5P + CO2 | #ABCDEF => #BCDEF + #A |
v6 | transketolase | Shewmr4_0773 | S7P + GAP <=> P5P + P5P | #abcdefg + #ABC <=> #cdefg + #abABC |
v7 | transaldolase | Shewmr4_2967 | S7P + GAP <=> F6P + E4P | #abcdefg + #ABC <=> #abcABC + #defg |
v8 | transketolase | Shewmr4_0773 | F6P + GAP <=> P5P + E4P | #abcdef + #ABC <=> #abABC+ #cdef |
v9 | glyceraldehy-3-phosphate dehydrogenase | Shewmr4_0536 | GAP <=> 1,3-BPG | #ABC <=> #ABC |
phosphoglycerate kinase | Shewmr4_0775 | 1,3-BPG <=> 3PG | #ABC <=> #ABC | |
phosphoglyceromutase | Shewmr4_0045 | 3PG <=> 2PG | #ABC <=> #ABC | |
enolase | Shewmr4_1115 | 2PG <=> PEP | #ABC <=> #ABC | |
v10 | pyruvate kinase | Shewmr4_2048 | PEP => PYR | #ABC => #ABC |
v11 | phosphoenolpyruvate carboxylase | Shewmr4_3710 | PEP + CO2 => OAA | #ABC + #D => #ABCD |
v12 | phosphoenolpyruvate carboxykinase | Shewmr4_0152 | OAA => PEP + CO2 | #ABCD => #ABC + #D |
v13 | NAD-dependent malic enzyme | Shewmr4_3188 | MAL <=> PYR + CO2 | #ABCD <=> #ABC + #D |
NADP-dependent malic enzyme | Shewmr4_3492 | |||
v14 | pyruvate dehydrogenase subunit E1 | Shewmr4_0428 | PYR =>ACoA + CO2 | #ABC=>#BC + #A |
pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase | Shewmr4_0429 | |||
dihydrolipoamide dehydrogenase | Shewmr4_0430 | |||
v15 | type Ⅱ citrate synthase | Shewmr4_1630 | ACoA + OAA => ICT | #abcd+#AB => #dcbaBA |
bifunctional aconitate hydratase | Shewmr4_0437 | |||
v16 | isocitrate dehydrogenase, NADP-dependent | Shewmr4_1606 | ICT => AKG + CO2 | #abcdef => #abcef + #d |
isocitrate dehydrogenase [NAD] | Shewmr4_2720 | |||
v17 | 2-oxoglutarate dehydrogenase E1 component | Shewmr4_1635 | AKG => FUM + CO2 | #ABCDE => #BCDE+#A |
dihydrolipoamide dehydrogenase | Shewmr4_0430 | |||
2-oxoglutarate dehydrogenase E2 component | Shewmr4_1636 | |||
succinyl-CoA synthetase subunit beta | Shewmr4_1637 | |||
fumarate reductase cytochrome b-556 subunit | Shewmr4_0402 | |||
fumarase | Shewmr4_2124 | FUM <=> MAL | #ABCD <=> #ABCD #ABCD => #DCBA | |
v18 | malate dehydrogenase | Shewmr4_3339 | MAL <=> OAA | #ABCD <=> #ABCD |
v19 | biomass synthesis | |||
v20 | acetate formation | ACoA => Ace | ||
v21 | EPS synthesis | G6P => EPS |
Table 4 Central metabolic network in Shewanella sp. MR-4 with the stoichiometric reactions and the corresponding carbon atom transitions
反应编号 (Reaction No.) | 酶 (Enzyme) | 基因 (Gene) | 反应化学计量学 (Stoichiometric reaction) | 碳原子转换 (Carbon atom transition) |
---|---|---|---|---|
v1 | glucokinase | Shewmr4_1763 | Glucose => G6P | #ABCDEF => #ABCDEF |
v2 | glucose-6-phosphate 1-dehydrogenase | Shewmr4_2046 | G6P => 6PG | #ABCDEF => #ABCDEF |
6-phosphogluconolactonase | Shewmr4_2045 | |||
v3 | phosphogluconate dehydratase | Shewmr4_2044 | 6PG => KDPG | #ABCDEF => #ABCDEF |
2-keto-3-deoxy-phosphogluconate aldolase | Shewmr4_2043 | KDPG => GAP + PYR | #ABCDEF => #DEF + #ABC | |
v4 | glucose-6-phosphate isomerase | Shewmr4_2968 | G6P <=> F6P | #ABCDEF <=> #ABCDEF |
v5 | 6-phosphogluconate dehydrogenase | Shewmr4_1609 | 6PG => P5P + CO2 | #ABCDEF => #BCDEF + #A |
v6 | transketolase | Shewmr4_0773 | S7P + GAP <=> P5P + P5P | #abcdefg + #ABC <=> #cdefg + #abABC |
v7 | transaldolase | Shewmr4_2967 | S7P + GAP <=> F6P + E4P | #abcdefg + #ABC <=> #abcABC + #defg |
v8 | transketolase | Shewmr4_0773 | F6P + GAP <=> P5P + E4P | #abcdef + #ABC <=> #abABC+ #cdef |
v9 | glyceraldehy-3-phosphate dehydrogenase | Shewmr4_0536 | GAP <=> 1,3-BPG | #ABC <=> #ABC |
phosphoglycerate kinase | Shewmr4_0775 | 1,3-BPG <=> 3PG | #ABC <=> #ABC | |
phosphoglyceromutase | Shewmr4_0045 | 3PG <=> 2PG | #ABC <=> #ABC | |
enolase | Shewmr4_1115 | 2PG <=> PEP | #ABC <=> #ABC | |
v10 | pyruvate kinase | Shewmr4_2048 | PEP => PYR | #ABC => #ABC |
v11 | phosphoenolpyruvate carboxylase | Shewmr4_3710 | PEP + CO2 => OAA | #ABC + #D => #ABCD |
v12 | phosphoenolpyruvate carboxykinase | Shewmr4_0152 | OAA => PEP + CO2 | #ABCD => #ABC + #D |
v13 | NAD-dependent malic enzyme | Shewmr4_3188 | MAL <=> PYR + CO2 | #ABCD <=> #ABC + #D |
NADP-dependent malic enzyme | Shewmr4_3492 | |||
v14 | pyruvate dehydrogenase subunit E1 | Shewmr4_0428 | PYR =>ACoA + CO2 | #ABC=>#BC + #A |
pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase | Shewmr4_0429 | |||
dihydrolipoamide dehydrogenase | Shewmr4_0430 | |||
v15 | type Ⅱ citrate synthase | Shewmr4_1630 | ACoA + OAA => ICT | #abcd+#AB => #dcbaBA |
bifunctional aconitate hydratase | Shewmr4_0437 | |||
v16 | isocitrate dehydrogenase, NADP-dependent | Shewmr4_1606 | ICT => AKG + CO2 | #abcdef => #abcef + #d |
isocitrate dehydrogenase [NAD] | Shewmr4_2720 | |||
v17 | 2-oxoglutarate dehydrogenase E1 component | Shewmr4_1635 | AKG => FUM + CO2 | #ABCDE => #BCDE+#A |
dihydrolipoamide dehydrogenase | Shewmr4_0430 | |||
2-oxoglutarate dehydrogenase E2 component | Shewmr4_1636 | |||
succinyl-CoA synthetase subunit beta | Shewmr4_1637 | |||
fumarate reductase cytochrome b-556 subunit | Shewmr4_0402 | |||
fumarase | Shewmr4_2124 | FUM <=> MAL | #ABCD <=> #ABCD #ABCD => #DCBA | |
v18 | malate dehydrogenase | Shewmr4_3339 | MAL <=> OAA | #ABCD <=> #ABCD |
v19 | biomass synthesis | |||
v20 | acetate formation | ACoA => Ace | ||
v21 | EPS synthesis | G6P => EPS |
Fig. 1 Bioreaction network of central carbon metabolism of Shewanella sp. MR-4(Dashed arrows indicate reactions or pathways identified to be inactive in this study. Double-headed arrows indicate reactions assumed to be reversible)
代谢物 (Metabolite) | Shewanella sp. MR-4在总池中的比例 (Percentage of total pool in Shewanella sp. MR-4) | |||
---|---|---|---|---|
硝酸盐(nitrate) | Fe | DMSO | 延胡索酸(fumarate) | |
PEP from PP pathway (ub) | 2.2 % | 3.2 % | 6.7 % | 1.9 % |
PEP from OAA (ub) | 2.7 % | 2.7 % | 6.7 % | 0.0 % |
PYR from OAA (ub) | 7.2 % | 11.8 % | 29.8 % | 0.5 % |
PYR from OAA (lb) | 3.4 % | 9.7 % | 11.7 % | 0.5 % |
OAA from PEP | 0.0 % | 0.0 % | 80.7 % | 93.1 % |
MAL from PYR | 33.5 % | 84.3 % | 0.0 % | 0.0 % |
OAA from glyoxylate shunt | 0.0 % | 0.0 % | 0.0 % | 0.0 % |
Ser from Gly | 0.0 % | 28.6 % | 18.6 % | 23.0 % |
Gly from Ser | 84.2 % | 77.0 % | 100.0 % | 86.1 % |
Table 5 Origins of metabolic intermediates determined by flux ratio analysis
代谢物 (Metabolite) | Shewanella sp. MR-4在总池中的比例 (Percentage of total pool in Shewanella sp. MR-4) | |||
---|---|---|---|---|
硝酸盐(nitrate) | Fe | DMSO | 延胡索酸(fumarate) | |
PEP from PP pathway (ub) | 2.2 % | 3.2 % | 6.7 % | 1.9 % |
PEP from OAA (ub) | 2.7 % | 2.7 % | 6.7 % | 0.0 % |
PYR from OAA (ub) | 7.2 % | 11.8 % | 29.8 % | 0.5 % |
PYR from OAA (lb) | 3.4 % | 9.7 % | 11.7 % | 0.5 % |
OAA from PEP | 0.0 % | 0.0 % | 80.7 % | 93.1 % |
MAL from PYR | 33.5 % | 84.3 % | 0.0 % | 0.0 % |
OAA from glyoxylate shunt | 0.0 % | 0.0 % | 0.0 % | 0.0 % |
Ser from Gly | 0.0 % | 28.6 % | 18.6 % | 23.0 % |
Gly from Ser | 84.2 % | 77.0 % | 100.0 % | 86.1 % |
Fig. 2 Intracellular metabolic flux distributions in Shewanella sp. MR-4 with (a) nitrate, Fe3+, (b) fumarate or DMSO as terminal electron acceptor[The number beside a metabolic reaction denotes its net flux, which was normalized by specific rate of glucose uptake](a) top, nitrate; bottom, Fe3+; (b) Top, fumarate; bottom, DMSO
Fig. 3 NADH balancing based on metabolic flux distributions(NADH is formed by the PDH complex, the TCA cycle and biomass synthesis. NADH was consumed via electron acceptor reduction. NADH consumption rate cannot be determined with nitrate as terminal electron acceptor)
片段 Fragments | 碳原子 C-atom | 前体 precursor | 前体的碳原子 C-atom of precursor | m | m+1 | m+2 | m+3 | m+4 | m+5 | m+6 | m+7 | m+8 | m+9 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
硝酸盐 Nitrate (U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.7771 | 0.0586 | 0.0285 | 0.1358 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.8027 | 0.0475 | 0.1497 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.6223 | 0.1759 | 0.1167 | 0.0677 | 0.0173 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6698 | 0.1857 | 0.1123 | 0.0321 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.7723 | 0.1108 | 0.1169 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5469 | 0.1780 | 0.1864 | 0.0622 | 0.0207 | 0.0059 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.5910 | 0.1856 | 0.1753 | 0.0357 | 0.0124 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.7247 | 0.1880 | 0.0874 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8083 | 0.0612 | 0.1305 | ||||||||
M_gly_085 | 1 | 1 | 0.8354 | 0.1646 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.5165 | 0.1481 | 0.2009 | 0.0912 | 0.0432 | 0.0000 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.5553 | 0.1702 | 0.1861 | 0.0620 | 0.0202 | 0.0063 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.5395 | 0.0884 | 0.2743 | 0.0305 | 0.0650 | 0.0023 | 0.0000 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.5507 | 0.1606 | 0.2028 | 0.0551 | 0.0254 | 0.0054 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.5194 | 0.1706 | 0.1497 | 0.0992 | 0.0416 | 0.0145 | 0.0049 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.5333 | 0.1854 | 0.1893 | 0.0636 | 0.0204 | 0.0080 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.6538 | 0.2656 | 0.0807 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6959 | 0.2209 | 0.0353 | 0.0297 | 0.0137 | 0.0045 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.7233 | 0.2133 | 0.0389 | 0.0216 | 0.0030 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4987 | 0.1004 | 0.0907 | 0.1524 | 0.0706 | 0.0388 | 0.0302 | 0.0125 | 0.0032 | 0.0026 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.5004 | 0.0965 | 0.1771 | 0.0872 | 0.0709 | 0.0361 | 0.0240 | 0.0046 | 0.0031 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.8280 | 0.0179 | 0.1541 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.3803 | 0.1470 | 0.1487 | 0.0851 | 0.1094 | 0.0456 | 0.0586 | 0.0252 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5628 | 0.1626 | 0.1632 | 0.0812 | 0.0226 | 0.0076 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.6054 | 0.1808 | 0.1667 | 0.0343 | 0.0127 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.7811 | 0.0469 | 0.0218 | 0.1502 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.7845 | 0.0653 | 0.1501 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.8107 | 0.0185 | 0.1708 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6021 | 0.1815 | 0.1296 | 0.0636 | 0.0233 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6554 | 0.1878 | 0.1305 | 0.0262 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.6432 | 0.2531 | 0.1037 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.6424 | 0.0767 | 0.1348 | 0.1113 | 0.0118 | 0.0229 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.6590 | 0.0690 | 0.2307 | 0.0153 | 0.0260 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.7932 | 0.0604 | 0.1464 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4878 | 0.1020 | 0.1013 | 0.1531 | 0.0730 | 0.0398 | 0.0269 | 0.0128 | 0.0032 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4889 | 0.1005 | 0.1861 | 0.0923 | 0.0695 | 0.0325 | 0.0252 | 0.0037 | 0.0014 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.8181 | 0.0223 | 0.1596 | |||||||
Fe (U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.6653 | 0.0687 | 0.0481 | 0.2180 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.7027 | 0.0516 | 0.2457 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.5139 | 0.1980 | 0.0784 | 0.1563 | 0.0534 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5856 | 0.1580 | 0.1399 | 0.1165 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.6452 | 0.1865 | 0.1683 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.4265 | 0.1428 | 0.2328 | 0.1277 | 0.0408 | 0.0294 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.4863 | 0.1174 | 0.3028 | 0.0394 | 0.0541 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.6145 | 0.2689 | 0.1166 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.7073 | 0.1256 | 0.1672 | ||||||||
M_gly_085 | 1 | 1 | 0.7732 | 0.2268 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.3812 | 0.1562 | 0.1741 | 0.1614 | 0.0538 | 0.0524 | 0.0208 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.4351 | 0.1340 | 0.2355 | 0.1232 | 0.0412 | 0.0311 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.3875 | 0.0690 | 0.3376 | 0.0590 | 0.1262 | 0.0072 | 0.0134 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.3941 | 0.1712 | 0.2546 | 0.1107 | 0.0524 | 0.0169 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.3937 | 0.1582 | 0.1451 | 0.1789 | 0.0628 | 0.0405 | 0.0209 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.4262 | 0.1373 | 0.2278 | 0.1236 | 0.0429 | 0.0421 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.5390 | 0.3428 | 0.1181 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.3944 | 0.2637 | 0.1093 | 0.1351 | 0.0809 | 0.0167 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.4615 | 0.2480 | 0.1384 | 0.1233 | 0.0288 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.3504 | 0.0882 | 0.1024 | 0.1830 | 0.0992 | 0.0689 | 0.0587 | 0.0279 | 0.0108 | 0.0106 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.3535 | 0.0849 | 0.2022 | 0.1126 | 0.1116 | 0.0599 | 0.0527 | 0.0115 | 0.0110 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.7337 | 0.0211 | 0.2451 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.2751 | 0.1470 | 0.1734 | 0.1093 | 0.1352 | 0.0607 | 0.0709 | 0.0284 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5409 | 0.1403 | 0.1547 | 0.1071 | 0.0337 | 0.0233 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.5292 | 0.0996 | 0.2819 | 0.0382 | 0.0511 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.6402 | 0.1096 | 0.0697 | 0.1805 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.6570 | 0.1600 | 0.1830 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.7130 | 0.0548 | 0.2321 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.5067 | 0.1940 | 0.0814 | 0.1609 | 0.0571 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5781 | 0.1512 | 0.1539 | 0.1168 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.5460 | 0.2992 | 0.1549 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5007 | 0.0748 | 0.1878 | 0.1625 | 0.0208 | 0.0535 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.5210 | 0.0661 | 0.3278 | 0.0241 | 0.0611 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.6948 | 0.0725 | 0.2327 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.3363 | 0.0843 | 0.1144 | 0.1806 | 0.1059 | 0.0691 | 0.0615 | 0.0273 | 0.0104 | 0.0102 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.3358 | 0.0837 | 0.2071 | 0.1196 | 0.1076 | 0.0653 | 0.0558 | 0.0121 | 0.0131 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.7298 | 0.0199 | 0.2504 | |||||||
延胡索酸 Fumarate(U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.7549 | 0.0769 | 0.0532 | 0.1149 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.8079 | 0.0369 | 0.1553 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.8566 | 0.0824 | 0.0227 | 0.0326 | 0.0057 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8834 | 0.0649 | 0.0371 | 0.0146 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.9028 | 0.0649 | 0.0322 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.7139 | 0.0853 | 0.1649 | 0.0262 | 0.0071 | 0.0027 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.7417 | 0.0774 | 0.1629 | 0.0098 | 0.0083 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.8524 | 0.1177 | 0.0300 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8221 | 0.0534 | 0.1245 | ||||||||
M_gly_085 | 1 | 1 | 0.8500 | 0.1500 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.6803 | 0.1019 | 0.1614 | 0.0377 | 0.0149 | 0.0038 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.6985 | 0.0910 | 0.1743 | 0.0255 | 0.0079 | 0.0027 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.5413 | 0.0739 | 0.2895 | 0.0299 | 0.0604 | 0.0006 | 0.0045 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.5449 | 0.1569 | 0.2142 | 0.0551 | 0.0240 | 0.0048 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.6674 | 0.1133 | 0.1150 | 0.0846 | 0.0119 | 0.0062 | 0.0016 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3&4,3,2 | 0.6967 | 0.0906 | 0.1707 | 0.0279 | 0.0100 | 0.0040 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.7318 | 0.2435 | 0.0247 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6959 | 0.2209 | 0.0353 | 0.0297 | 0.0137 | 0.0045 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.7233 | 0.2133 | 0.0389 | 0.0216 | 0.0030 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4690 | 0.1018 | 0.1024 | 0.1654 | 0.0744 | 0.0383 | 0.0293 | 0.0131 | 0.0033 | 0.0030 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4716 | 0.1001 | 0.1908 | 0.0929 | 0.0788 | 0.0329 | 0.0263 | 0.0036 | 0.0029 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.8166 | 0.0181 | 0.1653 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.3993 | 0.1696 | 0.1489 | 0.0949 | 0.0984 | 0.0384 | 0.0504 | |||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6515 | 0.0727 | 0.1395 | 0.1168 | 0.0162 | 0.0033 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.7504 | 0.0787 | 0.1576 | 0.0060 | 0.0073 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.7535 | 0.0701 | 0.0430 | 0.1334 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.7630 | 0.1045 | 0.1325 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.7998 | 0.0287 | 0.1715 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.8495 | 0.0858 | 0.0229 | 0.0346 | 0.0072 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8791 | 0.0609 | 0.0440 | 0.0160 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.7799 | 0.1869 | 0.0332 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.6138 | 0.0911 | 0.1594 | 0.1045 | 0.0120 | 0.0193 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.6486 | 0.0641 | 0.2490 | 0.0122 | 0.0261 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.7650 | 0.0993 | 0.1357 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4588 | 0.1002 | 0.1068 | 0.1695 | 0.0761 | 0.0369 | 0.0308 | 0.0138 | 0.0029 | 0.0043 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4680 | 0.0996 | 0.1933 | 0.0916 | 0.0816 | 0.0338 | 0.0243 | 0.0042 | 0.0037 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.8064 | 0.0214 | 0.1722 | |||||||
DMSO(U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.7596 | 0.0505 | 0.0361 | 0.1538 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.7778 | 0.0509 | 0.1713 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.6192 | 0.1729 | 0.0738 | 0.1092 | 0.0249 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6709 | 0.1582 | 0.1114 | 0.0596 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.7382 | 0.1339 | 0.1279 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5272 | 0.1559 | 0.2052 | 0.0792 | 0.0224 | 0.0102 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.5823 | 0.1361 | 0.2267 | 0.0291 | 0.0258 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.6949 | 0.2244 | 0.0807 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.7738 | 0.0761 | 0.1501 | ||||||||
M_gly_085 | 1 | 1 | 0.8112 | 0.1888 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.5000 | 0.1680 | 0.1581 | 0.1163 | 0.0465 | 0.0112 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.5293 | 0.1528 | 0.2062 | 0.0789 | 0.0209 | 0.0119 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.4933 | 0.1014 | 0.3028 | 0.0354 | 0.0606 | 0.0064 | 0.0000 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.5086 | 0.1741 | 0.2230 | 0.0623 | 0.0258 | 0.0061 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.5194 | 0.1706 | 0.1497 | 0.0992 | 0.0416 | 0.0145 | 0.0049 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.5333 | 0.1854 | 0.1893 | 0.0636 | 0.0204 | 0.0080 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.6538 | 0.2656 | 0.0807 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.5209 | 0.2351 | 0.0858 | 0.1153 | 0.0357 | 0.0073 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.5631 | 0.2369 | 0.1186 | 0.0545 | 0.0269 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3& 1, 2, 3, 4 | 0.4415 | 0.1047 | 0.1088 | 0.1722 | 0.0743 | 0.0451 | 0.0320 | 0.0133 | 0.0049 | 0.0032 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4505 | 0.1030 | 0.1953 | 0.1010 | 0.0781 | 0.0390 | 0.0256 | 0.0044 | 0.0032 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.8022 | 0.0251 | 0.1727 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.3191 | 0.1447 | 0.1799 | 0.0898 | 0.1191 | 0.0467 | 0.0694 | 0.0313 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6670 | 0.1257 | 0.0716 | 0.0936 | 0.0306 | 0.0115 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.6131 | 0.1116 | 0.2324 | 0.0130 | 0.0299 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.7470 | 0.0797 | 0.0484 | 0.1249 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.7718 | 0.1342 | 0.0940 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.7695 | 0.0896 | 0.1410 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6153 | 0.1799 | 0.0688 | 0.1098 | 0.0262 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6649 | 0.1601 | 0.1175 | 0.0575 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.6324 | 0.2705 | 0.0970 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.6111 | 0.0749 | 0.1489 | 0.1245 | 0.0132 | 0.0274 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.6224 | 0.0756 | 0.2601 | 0.0144 | 0.0274 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.7795 | 0.0448 | 0.1757 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3& 1, 2, 3, 4 | 0.4346 | 0.1068 | 0.1137 | 0.1947 | 0.0657 | 0.0496 | 0.0234 | 0.0107 | 0.0000 | 0.0008 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4182 | 0.0981 | 0.1878 | 0.1259 | 0.0942 | 0.0459 | 0.0287 | 0.0000 | 0.0013 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.7929 | 0.0251 | 0.1821 | |||||||
硝酸盐 Nitrate(1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5398 | 0.4523 | 0.0079 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9793 | 0.0207 | 0.0000 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.7515 | 0.1998 | 0.0485 | 0.0000 | 0.0002 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8524 | 0.1461 | 0.0015 | 0.0000 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.8451 | 0.1546 | 0.0003 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.8287 | 0.1641 | 0.0068 | 0.0000 | 0.0003 | 0.0000 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9262 | 0.0711 | 0.0020 | 0.0004 | 0.0004 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.8445 | 0.1554 | 0.0000 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8995 | 0.0979 | 0.0026 | ||||||||
M_gly_085 | 1 | 1 | 0.9867 | 0.0133 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.7233 | 0.2029 | 0.0583 | 0.0103 | 0.0041 | 0.0011 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.8226 | 0.1686 | 0.0083 | 0.0002 | 0.0002 | 0.0002 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9312 | 0.0618 | 0.0010 | 0.0054 | 0.0005 | 0.0000 | 0.0001 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9350 | 0.0645 | 0.0003 | 0.0002 | 0.0001 | 0.0000 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.4759 | 0.3053 | 0.1717 | 0.0000 | 0.0246 | 0.0083 | 0.0142 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.2194 | 0.0498 | 0.1072 | 0.1872 | 0.3795 | 0.0569 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.6946 | 0.2732 | 0.0322 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.7313 | 0.2028 | 0.0503 | 0.0086 | 0.0027 | 0.0042 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.8263 | 0.1619 | 0.0072 | 0.0032 | 0.0014 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8884 | 0.1044 | 0.0041 | 0.0009 | 0.0001 | 0.0007 | 0.0005 | 0.0005 | 0.0003 | 0.0002 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8961 | 0.0939 | 0.0041 | 0.0018 | 0.0006 | 0.0008 | 0.0007 | 0.0015 | 0.0004 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9771 | 0.0229 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7523 | 0.0947 | 0.0332 | 0.0085 | 0.0215 | 0.0207 | 0.0405 | 0.0286 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.8748 | 0.1149 | 0.0000 | 0.0042 | 0.0045 | 0.0016 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9366 | 0.0612 | 0.0011 | 0.0000 | 0.0010 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9605 | 0.0391 | 0.0000 | 0.0004 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9810 | 0.0190 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9667 | 0.0304 | 0.0029 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.7489 | 0.1979 | 0.0517 | 0.0007 | 0.0008 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8435 | 0.1479 | 0.0086 | 0.0000 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.7463 | 0.2252 | 0.0285 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5218 | 0.4572 | 0.0200 | 0.0009 | 0.0000 | 0.0000 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9458 | 0.0511 | 0.0027 | 0.0003 | 0.0002 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.5866 | 0.4116 | 0.0018 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8800 | 0.1030 | 0.0044 | 0.0000 | 0.0011 | 0.0004 | 0.0006 | 0.0006 | 0.0031 | 0.0068 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8890 | 0.0969 | 0.0014 | 0.0011 | 0.0011 | 0.0014 | 0.0002 | 0.0047 | 0.0042 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9779 | 0.0220 | 0.0001 | |||||||
Fe (1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5273 | 0.4640 | 0.0088 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9764 | 0.0230 | 0.0006 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.3476 | 0.4571 | 0.1910 | 0.0043 | 0.0000 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5600 | 0.4314 | 0.0086 | 0.0000 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.5890 | 0.4087 | 0.0023 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5616 | 0.4186 | 0.0195 | 0.0002 | 0.0000 | 0.0000 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.8990 | 0.0986 | 0.0020 | 0.0000 | 0.0004 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.6891 | 0.3048 | 0.0062 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.7664 | 0.2334 | 0.0002 | ||||||||
M_gly_085 | 1 | 1 | 0.9934 | 0.0066 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.3508 | 0.4485 | 0.1854 | 0.0134 | 0.0019 | 0.0000 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.5499 | 0.4265 | 0.0229 | 0.0000 | 0.0007 | 0.0000 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9468 | 0.0484 | 0.0017 | 0.0029 | 0.0000 | 0.0000 | 0.0002 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9339 | 0.0657 | 0.0001 | 0.0001 | 0.0002 | 0.0000 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/ 1,2,3&4,3,2 | 0.3053 | 0.4567 | 0.2213 | 0.0123 | 0.0005 | 0.0025 | 0.0014 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.4979 | 0.4162 | 0.0247 | 0.0080 | 0.0057 | 0.0474 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.8733 | 0.1267 | 0.0000 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.3424 | 0.4536 | 0.1957 | 0.0079 | 0.0000 | 0.0004 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.5530 | 0.4297 | 0.0165 | 0.0000 | 0.0008 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3& 1, 2, 3, 4 | 0.8883 | 0.1064 | 0.0045 | 0.0000 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0000 | 0.0000 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8976 | 0.0996 | 0.0021 | 0.0002 | 0.0000 | 0.0003 | 0.0001 | 0.0001 | 0.0000 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9859 | 0.0141 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7505 | 0.0973 | 0.0400 | 0.0097 | 0.0295 | 0.0126 | 0.0372 | 0.0232 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6058 | 0.3772 | 0.0109 | 0.0043 | 0.0011 | 0.0008 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.8992 | 0.0964 | 0.0039 | 0.0002 | 0.0004 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9242 | 0.0736 | 0.0021 | 0.0000 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9848 | 0.0152 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9275 | 0.0696 | 0.0029 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.3373 | 0.4592 | 0.2014 | 0.0021 | 0.0000 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5589 | 0.4272 | 0.0135 | 0.0004 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.5019 | 0.4845 | 0.0136 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5165 | 0.4582 | 0.0251 | 0.0000 | 0.0000 | 0.0002 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9413 | 0.0551 | 0.0033 | 0.0000 | 0.0003 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.5793 | 0.4195 | 0.0012 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8856 | 0.1102 | 0.0031 | 0.0000 | 0.0005 | 0.0005 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8974 | 0.0979 | 0.0021 | 0.0018 | 0.0000 | 0.0005 | 0.0002 | 0.0000 | 0.0000 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9857 | 0.0143 | 0.0000 | |||||||
延胡索酸 Fumarate (1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5836 | 0.4087 | 0.0077 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9776 | 0.0224 | 0.0000 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.9250 | 0.0653 | 0.0090 | 0.0000 | 0.0008 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9468 | 0.0532 | 0.0000 | 0.0000 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.9549 | 0.0441 | 0.0009 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.9232 | 0.0758 | 0.0000 | 0.0009 | 0.0000 | 0.0001 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9509 | 0.0490 | 0.0000 | 0.0000 | 0.0000 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.9143 | 0.0857 | 0.0000 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8501 | 0.1485 | 0.0014 | ||||||||
M_gly_085 | 1 | 1 | 0.9928 | 0.0072 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.8896 | 0.0983 | 0.0109 | 0.0000 | 0.0009 | 0.0000 | 0.0003 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.9197 | 0.0794 | 0.0003 | 0.0000 | 0.0005 | 0.0000 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9294 | 0.0674 | 0.0030 | 0.0000 | 0.0000 | 0.0000 | 0.0002 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9340 | 0.0657 | 0.0000 | 0.0000 | 0.0002 | 0.0001 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.7096 | 0.2448 | 0.0374 | 0.0065 | 0.0000 | 0.0000 | 0.0017 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.8280 | 0.1000 | 0.0134 | 0.0026 | 0.0091 | 0.0469 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.8531 | 0.1469 | 0.0000 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9177 | 0.0766 | 0.0000 | 0.0030 | 0.0014 | 0.0013 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.9272 | 0.0717 | 0.0000 | 0.0000 | 0.0011 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8943 | 0.0981 | 0.0068 | 0.0000 | 0.0000 | 0.0004 | 0.0001 | 0.0003 | 0.0000 | 0.0000 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8958 | 0.0995 | 0.0035 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0000 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9865 | 0.0135 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7512 | 0.0956 | 0.0420 | 0.0092 | 0.0288 | 0.0134 | 0.0346 | 0.0251 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.7781 | 0.0692 | 0.0009 | 0.1459 | 0.0060 | 0.0000 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9442 | 0.0556 | 0.0000 | 0.0000 | 0.0001 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9120 | 0.0857 | 0.0023 | 0.0000 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9798 | 0.0202 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9211 | 0.0789 | 0.0000 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9300 | 0.0609 | 0.0088 | 0.0000 | 0.0003 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9517 | 0.0464 | 0.0019 | 0.0000 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.8347 | 0.1521 | 0.0131 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5676 | 0.4133 | 0.0184 | 0.0000 | 0.0006 | 0.0001 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9485 | 0.0511 | 0.0000 | 0.0001 | 0.0003 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.6328 | 0.3672 | 0.0000 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8977 | 0.1009 | 0.0000 | 0.0007 | 0.0006 | 0.0001 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.9080 | 0.0882 | 0.0002 | 0.0018 | 0.0016 | 0.0000 | 0.0000 | 0.0000 | 0.0001 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9834 | 0.0166 | 0.0000 | |||||||
DMSO (1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5872 | 0.4050 | 0.0078 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9792 | 0.0208 | 0.0000 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.9336 | 0.0586 | 0.0075 | 0.0002 | 0.0000 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9558 | 0.0440 | 0.0000 | 0.0002 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.9664 | 0.0336 | 0.0000 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.9268 | 0.0723 | 0.0000 | 0.0009 | 0.0000 | 0.0000 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9477 | 0.0500 | 0.0000 | 0.0023 | 0.0000 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.8997 | 0.1003 | 0.0000 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8728 | 0.1263 | 0.0010 | ||||||||
M_gly_085 | 1 | 1 | 0.9910 | 0.0090 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.8904 | 0.0931 | 0.0158 | 0.0000 | 0.0000 | 0.0003 | 0.0003 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.9199 | 0.0786 | 0.0011 | 0.0002 | 0.0002 | 0.0000 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9369 | 0.0608 | 0.0000 | 0.0016 | 0.0005 | 0.0000 | 0.0002 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9308 | 0.0688 | 0.0000 | 0.0000 | 0.0003 | 0.0000 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.7104 | 0.2644 | 0.0183 | 0.0053 | 0.0013 | 0.0002 | 0.0000 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3&4,3,2 | 0.8848 | 0.0838 | 0.0017 | 0.0073 | 0.0019 | 0.0205 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.8678 | 0.1322 | 0.0000 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9140 | 0.0749 | 0.0080 | 0.0024 | 0.0000 | 0.0007 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.9399 | 0.0595 | 0.0005 | 0.0000 | 0.0000 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8961 | 0.0999 | 0.0018 | 0.0016 | 0.0000 | 0.0001 | 0.0003 | 0.0001 | 0.0000 | 0.0000 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8960 | 0.0987 | 0.0045 | 0.0000 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0000 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9835 | 0.0165 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7579 | 0.1032 | 0.0376 | 0.0077 | 0.0256 | 0.0115 | 0.0368 | 0.0197 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6492 | 0.0545 | 0.0009 | 0.2896 | 0.0058 | 0.0000 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9422 | 0.0553 | 0.0000 | 0.0024 | 0.0001 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9195 | 0.0803 | 0.0000 | 0.0002 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9858 | 0.0142 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9235 | 0.0728 | 0.0036 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9335 | 0.0616 | 0.0039 | 0.0004 | 0.0006 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9571 | 0.0400 | 0.0000 | 0.0028 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.8412 | 0.1503 | 0.0085 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5633 | 0.4172 | 0.0193 | 0.0000 | 0.0000 | 0.0002 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9400 | 0.0552 | 0.0048 | 0.0000 | 0.0000 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.6248 | 0.3687 | 0.0065 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8903 | 0.1039 | 0.0056 | 0.0001 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8916 | 0.1056 | 0.0000 | 0.0005 | 0.0016 | 0.0007 | 0.0000 | 0.0000 | 0.0001 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9859 | 0.0141 | 0.0000 |
(The symbols of fragments denote the cracking patterns of TBDMS-derivatized amino acids:(M-57)+,(M-85)+,(M-159)+ and f302 fragments)
片段 Fragments | 碳原子 C-atom | 前体 precursor | 前体的碳原子 C-atom of precursor | m | m+1 | m+2 | m+3 | m+4 | m+5 | m+6 | m+7 | m+8 | m+9 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
硝酸盐 Nitrate (U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.7771 | 0.0586 | 0.0285 | 0.1358 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.8027 | 0.0475 | 0.1497 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.6223 | 0.1759 | 0.1167 | 0.0677 | 0.0173 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6698 | 0.1857 | 0.1123 | 0.0321 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.7723 | 0.1108 | 0.1169 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5469 | 0.1780 | 0.1864 | 0.0622 | 0.0207 | 0.0059 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.5910 | 0.1856 | 0.1753 | 0.0357 | 0.0124 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.7247 | 0.1880 | 0.0874 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8083 | 0.0612 | 0.1305 | ||||||||
M_gly_085 | 1 | 1 | 0.8354 | 0.1646 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.5165 | 0.1481 | 0.2009 | 0.0912 | 0.0432 | 0.0000 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.5553 | 0.1702 | 0.1861 | 0.0620 | 0.0202 | 0.0063 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.5395 | 0.0884 | 0.2743 | 0.0305 | 0.0650 | 0.0023 | 0.0000 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.5507 | 0.1606 | 0.2028 | 0.0551 | 0.0254 | 0.0054 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.5194 | 0.1706 | 0.1497 | 0.0992 | 0.0416 | 0.0145 | 0.0049 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.5333 | 0.1854 | 0.1893 | 0.0636 | 0.0204 | 0.0080 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.6538 | 0.2656 | 0.0807 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6959 | 0.2209 | 0.0353 | 0.0297 | 0.0137 | 0.0045 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.7233 | 0.2133 | 0.0389 | 0.0216 | 0.0030 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4987 | 0.1004 | 0.0907 | 0.1524 | 0.0706 | 0.0388 | 0.0302 | 0.0125 | 0.0032 | 0.0026 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.5004 | 0.0965 | 0.1771 | 0.0872 | 0.0709 | 0.0361 | 0.0240 | 0.0046 | 0.0031 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.8280 | 0.0179 | 0.1541 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.3803 | 0.1470 | 0.1487 | 0.0851 | 0.1094 | 0.0456 | 0.0586 | 0.0252 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5628 | 0.1626 | 0.1632 | 0.0812 | 0.0226 | 0.0076 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.6054 | 0.1808 | 0.1667 | 0.0343 | 0.0127 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.7811 | 0.0469 | 0.0218 | 0.1502 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.7845 | 0.0653 | 0.1501 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.8107 | 0.0185 | 0.1708 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6021 | 0.1815 | 0.1296 | 0.0636 | 0.0233 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6554 | 0.1878 | 0.1305 | 0.0262 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.6432 | 0.2531 | 0.1037 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.6424 | 0.0767 | 0.1348 | 0.1113 | 0.0118 | 0.0229 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.6590 | 0.0690 | 0.2307 | 0.0153 | 0.0260 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.7932 | 0.0604 | 0.1464 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4878 | 0.1020 | 0.1013 | 0.1531 | 0.0730 | 0.0398 | 0.0269 | 0.0128 | 0.0032 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4889 | 0.1005 | 0.1861 | 0.0923 | 0.0695 | 0.0325 | 0.0252 | 0.0037 | 0.0014 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.8181 | 0.0223 | 0.1596 | |||||||
Fe (U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.6653 | 0.0687 | 0.0481 | 0.2180 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.7027 | 0.0516 | 0.2457 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.5139 | 0.1980 | 0.0784 | 0.1563 | 0.0534 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5856 | 0.1580 | 0.1399 | 0.1165 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.6452 | 0.1865 | 0.1683 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.4265 | 0.1428 | 0.2328 | 0.1277 | 0.0408 | 0.0294 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.4863 | 0.1174 | 0.3028 | 0.0394 | 0.0541 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.6145 | 0.2689 | 0.1166 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.7073 | 0.1256 | 0.1672 | ||||||||
M_gly_085 | 1 | 1 | 0.7732 | 0.2268 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.3812 | 0.1562 | 0.1741 | 0.1614 | 0.0538 | 0.0524 | 0.0208 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.4351 | 0.1340 | 0.2355 | 0.1232 | 0.0412 | 0.0311 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.3875 | 0.0690 | 0.3376 | 0.0590 | 0.1262 | 0.0072 | 0.0134 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.3941 | 0.1712 | 0.2546 | 0.1107 | 0.0524 | 0.0169 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.3937 | 0.1582 | 0.1451 | 0.1789 | 0.0628 | 0.0405 | 0.0209 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.4262 | 0.1373 | 0.2278 | 0.1236 | 0.0429 | 0.0421 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.5390 | 0.3428 | 0.1181 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.3944 | 0.2637 | 0.1093 | 0.1351 | 0.0809 | 0.0167 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.4615 | 0.2480 | 0.1384 | 0.1233 | 0.0288 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.3504 | 0.0882 | 0.1024 | 0.1830 | 0.0992 | 0.0689 | 0.0587 | 0.0279 | 0.0108 | 0.0106 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.3535 | 0.0849 | 0.2022 | 0.1126 | 0.1116 | 0.0599 | 0.0527 | 0.0115 | 0.0110 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.7337 | 0.0211 | 0.2451 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.2751 | 0.1470 | 0.1734 | 0.1093 | 0.1352 | 0.0607 | 0.0709 | 0.0284 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5409 | 0.1403 | 0.1547 | 0.1071 | 0.0337 | 0.0233 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.5292 | 0.0996 | 0.2819 | 0.0382 | 0.0511 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.6402 | 0.1096 | 0.0697 | 0.1805 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.6570 | 0.1600 | 0.1830 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.7130 | 0.0548 | 0.2321 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.5067 | 0.1940 | 0.0814 | 0.1609 | 0.0571 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5781 | 0.1512 | 0.1539 | 0.1168 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.5460 | 0.2992 | 0.1549 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5007 | 0.0748 | 0.1878 | 0.1625 | 0.0208 | 0.0535 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.5210 | 0.0661 | 0.3278 | 0.0241 | 0.0611 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.6948 | 0.0725 | 0.2327 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.3363 | 0.0843 | 0.1144 | 0.1806 | 0.1059 | 0.0691 | 0.0615 | 0.0273 | 0.0104 | 0.0102 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.3358 | 0.0837 | 0.2071 | 0.1196 | 0.1076 | 0.0653 | 0.0558 | 0.0121 | 0.0131 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.7298 | 0.0199 | 0.2504 | |||||||
延胡索酸 Fumarate(U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.7549 | 0.0769 | 0.0532 | 0.1149 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.8079 | 0.0369 | 0.1553 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.8566 | 0.0824 | 0.0227 | 0.0326 | 0.0057 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8834 | 0.0649 | 0.0371 | 0.0146 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.9028 | 0.0649 | 0.0322 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.7139 | 0.0853 | 0.1649 | 0.0262 | 0.0071 | 0.0027 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.7417 | 0.0774 | 0.1629 | 0.0098 | 0.0083 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.8524 | 0.1177 | 0.0300 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8221 | 0.0534 | 0.1245 | ||||||||
M_gly_085 | 1 | 1 | 0.8500 | 0.1500 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.6803 | 0.1019 | 0.1614 | 0.0377 | 0.0149 | 0.0038 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.6985 | 0.0910 | 0.1743 | 0.0255 | 0.0079 | 0.0027 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.5413 | 0.0739 | 0.2895 | 0.0299 | 0.0604 | 0.0006 | 0.0045 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.5449 | 0.1569 | 0.2142 | 0.0551 | 0.0240 | 0.0048 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.6674 | 0.1133 | 0.1150 | 0.0846 | 0.0119 | 0.0062 | 0.0016 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3&4,3,2 | 0.6967 | 0.0906 | 0.1707 | 0.0279 | 0.0100 | 0.0040 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.7318 | 0.2435 | 0.0247 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6959 | 0.2209 | 0.0353 | 0.0297 | 0.0137 | 0.0045 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.7233 | 0.2133 | 0.0389 | 0.0216 | 0.0030 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4690 | 0.1018 | 0.1024 | 0.1654 | 0.0744 | 0.0383 | 0.0293 | 0.0131 | 0.0033 | 0.0030 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4716 | 0.1001 | 0.1908 | 0.0929 | 0.0788 | 0.0329 | 0.0263 | 0.0036 | 0.0029 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.8166 | 0.0181 | 0.1653 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.3993 | 0.1696 | 0.1489 | 0.0949 | 0.0984 | 0.0384 | 0.0504 | |||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6515 | 0.0727 | 0.1395 | 0.1168 | 0.0162 | 0.0033 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.7504 | 0.0787 | 0.1576 | 0.0060 | 0.0073 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.7535 | 0.0701 | 0.0430 | 0.1334 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.7630 | 0.1045 | 0.1325 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.7998 | 0.0287 | 0.1715 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.8495 | 0.0858 | 0.0229 | 0.0346 | 0.0072 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8791 | 0.0609 | 0.0440 | 0.0160 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.7799 | 0.1869 | 0.0332 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.6138 | 0.0911 | 0.1594 | 0.1045 | 0.0120 | 0.0193 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.6486 | 0.0641 | 0.2490 | 0.0122 | 0.0261 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.7650 | 0.0993 | 0.1357 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.4588 | 0.1002 | 0.1068 | 0.1695 | 0.0761 | 0.0369 | 0.0308 | 0.0138 | 0.0029 | 0.0043 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4680 | 0.0996 | 0.1933 | 0.0916 | 0.0816 | 0.0338 | 0.0243 | 0.0042 | 0.0037 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.8064 | 0.0214 | 0.1722 | |||||||
DMSO(U-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.7596 | 0.0505 | 0.0361 | 0.1538 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.7778 | 0.0509 | 0.1713 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.6192 | 0.1729 | 0.0738 | 0.1092 | 0.0249 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6709 | 0.1582 | 0.1114 | 0.0596 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.7382 | 0.1339 | 0.1279 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5272 | 0.1559 | 0.2052 | 0.0792 | 0.0224 | 0.0102 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.5823 | 0.1361 | 0.2267 | 0.0291 | 0.0258 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.6949 | 0.2244 | 0.0807 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.7738 | 0.0761 | 0.1501 | ||||||||
M_gly_085 | 1 | 1 | 0.8112 | 0.1888 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.5227 | 0.1609 | 0.1080 | 0.1325 | 0.0357 | 0.0329 | 0.0073 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.5169 | 0.1612 | 0.2063 | 0.0689 | 0.0355 | 0.0112 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.7984 | 0.0361 | 0.1654 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.5000 | 0.1680 | 0.1581 | 0.1163 | 0.0465 | 0.0112 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.5293 | 0.1528 | 0.2062 | 0.0789 | 0.0209 | 0.0119 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.4933 | 0.1014 | 0.3028 | 0.0354 | 0.0606 | 0.0064 | 0.0000 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.5086 | 0.1741 | 0.2230 | 0.0623 | 0.0258 | 0.0061 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.5194 | 0.1706 | 0.1497 | 0.0992 | 0.0416 | 0.0145 | 0.0049 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.5333 | 0.1854 | 0.1893 | 0.0636 | 0.0204 | 0.0080 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.6538 | 0.2656 | 0.0807 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.5209 | 0.2351 | 0.0858 | 0.1153 | 0.0357 | 0.0073 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.5631 | 0.2369 | 0.1186 | 0.0545 | 0.0269 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3& 1, 2, 3, 4 | 0.4415 | 0.1047 | 0.1088 | 0.1722 | 0.0743 | 0.0451 | 0.0320 | 0.0133 | 0.0049 | 0.0032 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4505 | 0.1030 | 0.1953 | 0.1010 | 0.0781 | 0.0390 | 0.0256 | 0.0044 | 0.0032 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.8022 | 0.0251 | 0.1727 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.3191 | 0.1447 | 0.1799 | 0.0898 | 0.1191 | 0.0467 | 0.0694 | 0.0313 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6670 | 0.1257 | 0.0716 | 0.0936 | 0.0306 | 0.0115 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.6131 | 0.1116 | 0.2324 | 0.0130 | 0.0299 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.7470 | 0.0797 | 0.0484 | 0.1249 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.7718 | 0.1342 | 0.0940 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.7695 | 0.0896 | 0.1410 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.6153 | 0.1799 | 0.0688 | 0.1098 | 0.0262 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.6649 | 0.1601 | 0.1175 | 0.0575 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.6324 | 0.2705 | 0.0970 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.6111 | 0.0749 | 0.1489 | 0.1245 | 0.0132 | 0.0274 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.6224 | 0.0756 | 0.2601 | 0.0144 | 0.0274 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.7795 | 0.0448 | 0.1757 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3& 1, 2, 3, 4 | 0.4346 | 0.1068 | 0.1137 | 0.1947 | 0.0657 | 0.0496 | 0.0234 | 0.0107 | 0.0000 | 0.0008 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.4182 | 0.0981 | 0.1878 | 0.1259 | 0.0942 | 0.0459 | 0.0287 | 0.0000 | 0.0013 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.7929 | 0.0251 | 0.1821 | |||||||
硝酸盐 Nitrate(1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5398 | 0.4523 | 0.0079 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9793 | 0.0207 | 0.0000 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.7515 | 0.1998 | 0.0485 | 0.0000 | 0.0002 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8524 | 0.1461 | 0.0015 | 0.0000 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.8451 | 0.1546 | 0.0003 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.8287 | 0.1641 | 0.0068 | 0.0000 | 0.0003 | 0.0000 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9262 | 0.0711 | 0.0020 | 0.0004 | 0.0004 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.8445 | 0.1554 | 0.0000 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8995 | 0.0979 | 0.0026 | ||||||||
M_gly_085 | 1 | 1 | 0.9867 | 0.0133 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.7233 | 0.2029 | 0.0583 | 0.0103 | 0.0041 | 0.0011 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.8226 | 0.1686 | 0.0083 | 0.0002 | 0.0002 | 0.0002 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9312 | 0.0618 | 0.0010 | 0.0054 | 0.0005 | 0.0000 | 0.0001 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9350 | 0.0645 | 0.0003 | 0.0002 | 0.0001 | 0.0000 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.4759 | 0.3053 | 0.1717 | 0.0000 | 0.0246 | 0.0083 | 0.0142 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.2194 | 0.0498 | 0.1072 | 0.1872 | 0.3795 | 0.0569 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.6946 | 0.2732 | 0.0322 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.7313 | 0.2028 | 0.0503 | 0.0086 | 0.0027 | 0.0042 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.8263 | 0.1619 | 0.0072 | 0.0032 | 0.0014 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8884 | 0.1044 | 0.0041 | 0.0009 | 0.0001 | 0.0007 | 0.0005 | 0.0005 | 0.0003 | 0.0002 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8961 | 0.0939 | 0.0041 | 0.0018 | 0.0006 | 0.0008 | 0.0007 | 0.0015 | 0.0004 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9771 | 0.0229 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7523 | 0.0947 | 0.0332 | 0.0085 | 0.0215 | 0.0207 | 0.0405 | 0.0286 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.8748 | 0.1149 | 0.0000 | 0.0042 | 0.0045 | 0.0016 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9366 | 0.0612 | 0.0011 | 0.0000 | 0.0010 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9605 | 0.0391 | 0.0000 | 0.0004 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9810 | 0.0190 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9667 | 0.0304 | 0.0029 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.7489 | 0.1979 | 0.0517 | 0.0007 | 0.0008 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.8435 | 0.1479 | 0.0086 | 0.0000 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.7463 | 0.2252 | 0.0285 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5218 | 0.4572 | 0.0200 | 0.0009 | 0.0000 | 0.0000 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9458 | 0.0511 | 0.0027 | 0.0003 | 0.0002 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.5866 | 0.4116 | 0.0018 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8800 | 0.1030 | 0.0044 | 0.0000 | 0.0011 | 0.0004 | 0.0006 | 0.0006 | 0.0031 | 0.0068 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8890 | 0.0969 | 0.0014 | 0.0011 | 0.0011 | 0.0014 | 0.0002 | 0.0047 | 0.0042 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9779 | 0.0220 | 0.0001 | |||||||
Fe (1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5273 | 0.4640 | 0.0088 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9764 | 0.0230 | 0.0006 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.3476 | 0.4571 | 0.1910 | 0.0043 | 0.0000 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5600 | 0.4314 | 0.0086 | 0.0000 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.5890 | 0.4087 | 0.0023 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.5616 | 0.4186 | 0.0195 | 0.0002 | 0.0000 | 0.0000 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.8990 | 0.0986 | 0.0020 | 0.0000 | 0.0004 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.6891 | 0.3048 | 0.0062 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.7664 | 0.2334 | 0.0002 | ||||||||
M_gly_085 | 1 | 1 | 0.9934 | 0.0066 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.3508 | 0.4485 | 0.1854 | 0.0134 | 0.0019 | 0.0000 | 0.0000 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.5499 | 0.4265 | 0.0229 | 0.0000 | 0.0007 | 0.0000 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9468 | 0.0484 | 0.0017 | 0.0029 | 0.0000 | 0.0000 | 0.0002 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9339 | 0.0657 | 0.0001 | 0.0001 | 0.0002 | 0.0000 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/ 1,2,3&4,3,2 | 0.3053 | 0.4567 | 0.2213 | 0.0123 | 0.0005 | 0.0025 | 0.0014 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.4979 | 0.4162 | 0.0247 | 0.0080 | 0.0057 | 0.0474 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.8733 | 0.1267 | 0.0000 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.3424 | 0.4536 | 0.1957 | 0.0079 | 0.0000 | 0.0004 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.5530 | 0.4297 | 0.0165 | 0.0000 | 0.0008 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3& 1, 2, 3, 4 | 0.8883 | 0.1064 | 0.0045 | 0.0000 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0000 | 0.0000 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8976 | 0.0996 | 0.0021 | 0.0002 | 0.0000 | 0.0003 | 0.0001 | 0.0001 | 0.0000 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9859 | 0.0141 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7505 | 0.0973 | 0.0400 | 0.0097 | 0.0295 | 0.0126 | 0.0372 | 0.0232 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6058 | 0.3772 | 0.0109 | 0.0043 | 0.0011 | 0.0008 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.8992 | 0.0964 | 0.0039 | 0.0002 | 0.0004 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9242 | 0.0736 | 0.0021 | 0.0000 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9848 | 0.0152 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9275 | 0.0696 | 0.0029 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.3373 | 0.4592 | 0.2014 | 0.0021 | 0.0000 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.5589 | 0.4272 | 0.0135 | 0.0004 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.5019 | 0.4845 | 0.0136 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5165 | 0.4582 | 0.0251 | 0.0000 | 0.0000 | 0.0002 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9413 | 0.0551 | 0.0033 | 0.0000 | 0.0003 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.5793 | 0.4195 | 0.0012 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8856 | 0.1102 | 0.0031 | 0.0000 | 0.0005 | 0.0005 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8974 | 0.0979 | 0.0021 | 0.0018 | 0.0000 | 0.0005 | 0.0002 | 0.0000 | 0.0000 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9857 | 0.0143 | 0.0000 | |||||||
延胡索酸 Fumarate (1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5836 | 0.4087 | 0.0077 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9776 | 0.0224 | 0.0000 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.9250 | 0.0653 | 0.0090 | 0.0000 | 0.0008 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9468 | 0.0532 | 0.0000 | 0.0000 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.9549 | 0.0441 | 0.0009 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.9232 | 0.0758 | 0.0000 | 0.0009 | 0.0000 | 0.0001 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9509 | 0.0490 | 0.0000 | 0.0000 | 0.0000 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.9143 | 0.0857 | 0.0000 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8501 | 0.1485 | 0.0014 | ||||||||
M_gly_085 | 1 | 1 | 0.9928 | 0.0072 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.8896 | 0.0983 | 0.0109 | 0.0000 | 0.0009 | 0.0000 | 0.0003 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.9197 | 0.0794 | 0.0003 | 0.0000 | 0.0005 | 0.0000 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9294 | 0.0674 | 0.0030 | 0.0000 | 0.0000 | 0.0000 | 0.0002 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9340 | 0.0657 | 0.0000 | 0.0000 | 0.0002 | 0.0001 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.7096 | 0.2448 | 0.0374 | 0.0065 | 0.0000 | 0.0000 | 0.0017 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3& 4,3,2 | 0.8280 | 0.1000 | 0.0134 | 0.0026 | 0.0091 | 0.0469 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.8531 | 0.1469 | 0.0000 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9177 | 0.0766 | 0.0000 | 0.0030 | 0.0014 | 0.0013 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.9272 | 0.0717 | 0.0000 | 0.0000 | 0.0011 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8943 | 0.0981 | 0.0068 | 0.0000 | 0.0000 | 0.0004 | 0.0001 | 0.0003 | 0.0000 | 0.0000 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8958 | 0.0995 | 0.0035 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0000 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9865 | 0.0135 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7512 | 0.0956 | 0.0420 | 0.0092 | 0.0288 | 0.0134 | 0.0346 | 0.0251 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.7781 | 0.0692 | 0.0009 | 0.1459 | 0.0060 | 0.0000 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9442 | 0.0556 | 0.0000 | 0.0000 | 0.0001 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9120 | 0.0857 | 0.0023 | 0.0000 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9798 | 0.0202 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9211 | 0.0789 | 0.0000 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9300 | 0.0609 | 0.0088 | 0.0000 | 0.0003 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9517 | 0.0464 | 0.0019 | 0.0000 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.8347 | 0.1521 | 0.0131 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5676 | 0.4133 | 0.0184 | 0.0000 | 0.0006 | 0.0001 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9485 | 0.0511 | 0.0000 | 0.0001 | 0.0003 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.6328 | 0.3672 | 0.0000 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8977 | 0.1009 | 0.0000 | 0.0007 | 0.0006 | 0.0001 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.9080 | 0.0882 | 0.0002 | 0.0018 | 0.0016 | 0.0000 | 0.0000 | 0.0000 | 0.0001 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9834 | 0.0166 | 0.0000 | |||||||
DMSO (1-13C) | |||||||||||||
M_ala_057 | 1, 2, 3 | PYR | 1, 2, 3 | 0.5872 | 0.4050 | 0.0078 | 0.0000 | ||||||
M_ala_085 | 2, 3 | PYR | 2, 3 | 0.9792 | 0.0208 | 0.0000 | |||||||
M_asx_057 | 1, 2, 3, 4 | OAA | 1, 2, 3, 4 | 0.9336 | 0.0586 | 0.0075 | 0.0002 | 0.0000 | |||||
M_asx_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9558 | 0.0440 | 0.0000 | 0.0002 | ||||||
M_asx_302 | 1, 2 | OAA | 1, 2 | 0.9664 | 0.0336 | 0.0000 | |||||||
M_glx_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.9268 | 0.0723 | 0.0000 | 0.0009 | 0.0000 | 0.0000 | ||||
M_glx_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9477 | 0.0500 | 0.0000 | 0.0023 | 0.0000 | |||||
M_glx_302 | 1, 2 | AKG | 1, 2 | 0.8997 | 0.1003 | 0.0000 | |||||||
M_gly_057 | 1, 2 | 1, 2 | 0.8728 | 0.1263 | 0.0010 | ||||||||
M_gly_085 | 1 | 1 | 0.9910 | 0.0090 | |||||||||
M_his_057 | 1, 2, 3, 4, 5, 6 | R5P | 5, 4, 3, 2, 1 | 0.4425 | 0.4269 | 0.1183 | 0.0116 | 0.0002 | 0.0005 | 0.0000 | |||
M_his_159 | 2, 3, 4, 5, 6 | R5P | 4, 3, 2, 1 | 0.4720 | 0.4370 | 0.0742 | 0.0107 | 0.0044 | 0.0017 | ||||
M_his_302 | 1, 2 | R5P | 5, 4 | 0.8912 | 0.1088 | 0.0000 | |||||||
M_ile_015 | 1, 2, 3, 4, 5, 6 | OAA&PYR | 1,2,3,4&2,3 | 0.8904 | 0.0931 | 0.0158 | 0.0000 | 0.0000 | 0.0003 | 0.0003 | |||
M_ile_085 | 2, 3, 4, 5, 6 | OAA&PYR | 2,3,4&2,3 | 0.9199 | 0.0786 | 0.0011 | 0.0002 | 0.0002 | 0.0000 | ||||
M_leu_015 | 1, 2, 3, 4, 5, 6 | AcoA&PYR | 1,2&2,2,3,3 | 0.9369 | 0.0608 | 0.0000 | 0.0016 | 0.0005 | 0.0000 | 0.0002 | |||
M_leu_085 | 2, 3, 4, 5, 6 | AcoA&PYR | 2&2,2,3,3 | 0.9308 | 0.0688 | 0.0000 | 0.0000 | 0.0003 | 0.0000 | ||||
M_lys_057 | 1, 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 1,2,3,4&3,2/1,2,3&4,3,2 | 0.7104 | 0.2644 | 0.0183 | 0.0053 | 0.0013 | 0.0002 | 0.0000 | |||
M_lys_159 | 2, 3, 4, 5, 6 | OAA&PYR/PYR&OAA | 2,3,4&3,2/2,3&4,3,2 | 0.8848 | 0.0838 | 0.0017 | 0.0073 | 0.0019 | 0.0205 | ||||
M_lys_302 | 1,2 | OAA/PYR | 1,2/1,2 | 0.8678 | 0.1322 | 0.0000 | |||||||
M_met_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9140 | 0.0749 | 0.0080 | 0.0024 | 0.0000 | 0.0007 | ||||
M_met_085 | 2,3,4 | OAA | 2,3,4 | 0.9399 | 0.0595 | 0.0005 | 0.0000 | 0.0000 | |||||
M_phe_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8961 | 0.0999 | 0.0018 | 0.0016 | 0.0000 | 0.0001 | 0.0003 | 0.0001 | 0.0000 | 0.0000 |
M_phe_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8960 | 0.0987 | 0.0045 | 0.0000 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0000 | |
M_phe_302 | 1, 2 | PEP | 1, 2 | 0.9835 | 0.0165 | 0.0000 | |||||||
M_phe_sc | 3,4,5,6,7,8,9 | PEP&E4P | 3,2,3&1,2,3,4 | 0.7579 | 0.1032 | 0.0376 | 0.0077 | 0.0256 | 0.0115 | 0.0368 | 0.0197 | ||
M_pro_057 | 1, 2, 3, 4, 5 | AKG | 1, 2, 3, 4, 5 | 0.6492 | 0.0545 | 0.0009 | 0.2896 | 0.0058 | 0.0000 | ||||
M_pro_085 | 2, 3, 4, 5 | AKG | 2, 3, 4, 5 | 0.9422 | 0.0553 | 0.0000 | 0.0024 | 0.0001 | |||||
M_ser_057 | 1, 2, 3 | 1, 2, 3 | 0.9195 | 0.0803 | 0.0000 | 0.0002 | |||||||
M_ser_085 | 2, 3 | 2, 3 | 0.9858 | 0.0142 | 0.0000 | ||||||||
M_ser_302 | 1, 2 | 1, 2 | 0.9235 | 0.0728 | 0.0036 | ||||||||
M_thr_057 | 1,2,3,4 | OAA | 1,2,3,4 | 0.9335 | 0.0616 | 0.0039 | 0.0004 | 0.0006 | |||||
M_thr_085 | 2, 3, 4 | OAA | 2, 3, 4 | 0.9571 | 0.0400 | 0.0000 | 0.0028 | ||||||
M_thr_sc | 3,4 | OAA | 3,4 | 0.8412 | 0.1503 | 0.0085 | |||||||
M_val_057 | 1, 2, 3, 4, 5 | PYR | 1, 2, 2, 3, 3 | 0.5633 | 0.4172 | 0.0193 | 0.0000 | 0.0000 | 0.0002 | ||||
M_val_085 | 2, 3, 4, 5 | PYR | 2, 2, 3, 3 | 0.9400 | 0.0552 | 0.0048 | 0.0000 | 0.0000 | |||||
M_val_302 | 1, 2 | PYR | 1, 2 | 0.6248 | 0.3687 | 0.0065 | |||||||
M_tyr_057 | 1, 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 1, 2, 3, 2, 3&1, 2, 3, 4 | 0.8903 | 0.1039 | 0.0056 | 0.0001 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.0000 |
M_tyr_085 | 2, 3, 4, 5, 6, 7, 8, 9 | PEP&E4P | 2, 3, 2, 3&1, 2, 3, 4 | 0.8916 | 0.1056 | 0.0000 | 0.0005 | 0.0016 | 0.0007 | 0.0000 | 0.0000 | 0.0001 | |
M_tyr_302 | 1, 2 | PEP | 1, 2 | 0.9859 | 0.0141 | 0.0000 |
1 | MYERS C R, NEALSON K H. Bacterial Manganese reduction and growth with manganese oxide as the sole electron acceptor[J]. Science, 1988, 240(4857): 1319-1321. |
2 | LARSEN I, LITTLE B, NEALSON K H, et al. Manganite reduction by Shewanella putrefaciens MR-4[J]. American Mineralogist, 1998, 83(11/12): 1564-1572. |
3 | URRUTIA M M, RODEN E E, FREDRICKSON J K, et al. Microbial and surface chemistry controls on reduction of synthetic Fe(Ⅲ) oxide minerals by the dissimilatory iron-reducing bacterium Shewanella alga [J]. Geomicrobiology Journal, 1998, 15(4): 269-291. |
4 | CACCAVO F, BLAKEMORE R P, LOVLEY D R. A hydrogen-oxidizing, Fe(Ⅲ)-reducing microorganism from the Great Bay Estuary, New Hampshire[J]. Applied and Environmental Microbiology, 1992, 58(10): 3211-3216. |
5 | WIELINGA B, MIZUBA M M, HANSEL C M, et al. Iron promoted reduction of chromate by dissimilatory iron-reducing bacteria[J]. Environmental Science & Technology, 2001, 35(3): 522-527. |
6 | FARRENKOPF A M, DOLLHOPF M E, NI CHADHAIN S, et al. Reduction of iodate in seawater during Arabian Sea shipboard incubations and in laboratory cultures of the marine bacterium Shewanella putrefaciens strain MR-4[J]. Marine Chemistry, 1997, 57(3/4): 347-354. |
7 | WILDUNG R E, GORBY Y A, KRUPKA K M, et al. Effect of electron donor and solution chemistry on products of dissimilatory reduction of technetium by Shewanella putrefaciens [J]. Applied and Environmental Microbiology, 2000, 66(6): 2451-2460. |
8 | LLOYD J R, YONG P, MACASKIE L E. Biological reduction and removal of Np(Ⅴ) by two microorganisms[J]. Environmental Science & Technology, 2000, 34(7): 1297-1301. |
9 | SALTIKOV C W, WILDMAN R A JR, NEWMAN D K. Expression dynamics of arsenic respiration and detoxification in Shewanella sp. strain ANA-3[J]. Journal of Bacteriology, 2005, 187(21): 7390-7396. |
10 | BOUKHALFA H, ICOPINI G A, REILLY S D, et al. Plutonium(Ⅳ) reduction by the metal-reducing bacteria Geobacter metallireducens GS15 and Shewanella oneidensis MR1[J]. Applied and Environmental Microbiology, 2007, 73(18): 5897-5903. |
11 | KLONOWSKA A, HEULIN T, VERMEGLIO A. Selenite and tellurite reduction by Shewanella oneidensis [J]. Applied and Environmental Microbiology, 2005, 71(9): 5607-5609. |
12 | CARPENTIER W, SANDRA K, DE SMET I, et al. Microbial reduction and precipitation of vanadium by Shewanella oneidensis [J]. Applied and Environmental Microbiology, 2003, 69(6): 3636-3639. |
13 | PERRY K A, KOSTKA J E, LUTHER G W 3 RD,et al. Mediation of sulfur speciation by a Black Sea facultative anaerobe[J]. Science, 1993, 259(5096): 801-803. |
14 | KRAUSE B, NEALSON K H. Physiology and enzymology involved in denitrification by Shewanella putrefaciens [J]. Applied and Environmental Microbiology, 1997, 63(7): 2613-2618. |
15 | RINGØ E, STENBERG E, STRØM A R. Amino acid and lactate catabolism in trimethylamine oxide respiration of Alteromonas putrefaciens NCMB 1735[J]. Applied and Environmental Microbiology, 1984, 47(5): 1084-1089. |
16 | GRALNICK J A, VALI H, LIES D P, et al. Extracellular respiration of dimethyl sulfoxide by Shewanella oneidensis strain MR-1[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(12): 4669-4674. |
17 | MYERS C R, MYERS J M. Fumarate reductase is a soluble enzyme in anaerobically grown Shewanella putrefaciens MR-1[J]. FEMS Microbiology Letters, 1992, 98(1/2/3): 13-19. |
18 | ZHAO J S, MANNO D, BEAULIEU C, et al. Shewanella sediminis sp nov., a novel Na+-requiring and hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading bacterium from marine sediment[J]. International Journal of Systematic and Evolutionary Microbiology, 2005, 55(Pt 4): 1511-1520. |
19 | ZHAO J S, MANNO D, THIBOUTOT S, et al. Shewanella canadensis sp nov. and Shewanella atlantica sp. nov., manganese dioxide- and hexahydro-1,3,5-trinitro-1,3,5-triazine-reducing, psychrophilic marine bacteria[J]. International Journal of Systematic and Evolutionary Microbiology, 2007, 57(9): 2155-2162. |
20 | HAU H H, GRALNICK J A. Ecology and biotechnology of the genus Shewanella [J]. Annual Review of Microbiology, 2007, 61: 237-258. |
21 | 肖翔, 吴勇民, 徐灿灿, 等. 金属还原菌希瓦氏菌厌氧呼吸能力及其在环境修复中的研究进展[J]. 微生物学通报, 2012, 39(11): 1677-1686. |
XIAO X, WU Y M, XU C C, et al. Anaerobic respiratory capabilities of a metal-reducing microorganism Shewanella and its application in environmental remediation[J]. Microbiology China, 2012, 39(11): 1677-1686. | |
22 | TANG Y J J, HWANG J S, WEMMER D E, et al. Shewanella oneidensis MR-1 fluxome under various oxygen conditions[J]. Applied and Environmental Microbiology, 2007, 73(3): 718-729. |
23 | TANG Y J J, MARTIN H G, DEUTSCHBAUER A, et al. Invariability of central metabolic flux distribution in Shewanella oneidensis MR-1 under environmental or genetic perturbations[J]. Biotechnology Progress, 2009, 25(5): 1254-1259. |
24 | TANG Y J J, MARTIN H G, DEHAL P S, et al. Metabolic flux analysis of Shewanella spp. reveals evolutionary robustness in central carbon metabolism[J]. Biotechnology and Bioengineering, 2009, 102(4): 1161-1169. |
25 | TANG Y J J, MEADOWS A L, KIRBY J, et al. Anaerobic central metabolic pathways in Shewanella oneidensis MR-1 reinterpreted in the light of isotopic metabolite labeling[J]. Journal of Bacteriology, 2007, 189(3): 894-901. |
26 | STOOKEY L L. Ferrozine— a new spectrophotometric reagent for iron[J]. Analytical Chemistry, 1970, 42(7): 779-781. |
27 | ALEF K, KLEINER D. Rapid and sensitive determination of microbial activity in soils and in soil aggregates by dimethylsulfoxide reduction[J]. Biology and Fertility of Soils, 1989, 8(4): 349-355. |
28 | MASUKO T, MINAMI A, IWASAKI N, et al. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format[J]. Analytical Biochemistry, 2005, 339(1): 69-72. |
29 | ZAMBONI N, FENDT S M, RÜHL M, et al. 13C-based metabolic flux analysis[J]. Nature Protocols, 2009, 4(6): 878-892. |
30 | PINCHUK G E, HILL E A, GEYDEBREKHT O V, et al. Constraint-based model of Shewanella oneidensis MR-1 metabolism: a tool for data analysis and hypothesis generation[J]. PLoS Computational Biology, 2010, 6(6): e1000822. |
31 | NANCHEN A, FUHRER T, SAUER U. Determination of metabolic flux ratios from 13C-experiments and gas chromatography-mass spectrometry data[M/OL]//Methods in molecular biology: metabolomics. Totowa, NJ, USA: Humana Press. 2007, 358: 177-197 [2022-09-01]. . |
32 | AYYASAMY P M, CHUN S H, LEE S H. Desorption and dissolution of heavy metals from contaminated soil using Shewanella sp. (HN-41) amended with various carbon sources and synthetic soil organic matters[J]. Journal of Hazardous Materials, 2009, 161(2/3): 1095-1102. |
33 | ONG W K, VU T T, LOVENDAHL K N, et al. Comparisons of Shewanella strains based on genome annotations, modeling, and experiments[J]. BMC Systems Biology, 2014, 8: 31. |
34 | CRUZ-GARCÍA C, MURRAY A E, KLAPPENBACH J A, et al. Respiratory nitrate ammonification by Shewanella oneidensis MR-1[J]. Journal of Bacteriology, 2007, 189(2): 656-662. |
35 | INOHANA Y, KATSUYA S, KOGA R, et al. Shewanella algae relatives capable of generating electricity from acetate contribute to coastal-sediment microbial fuel cells treating complex organic matter[J]. Microbes and Environments, 2020, 35(2): ME19161. |
36 | REWATKAR P, GOEL S. Shewanella putrefaciens powered microfluidic microbial fuel cell with printed circuit board electrodes and soft-lithographic microchannel[J]. Chemosphere, 2022, 286: 131855. |
37 | DAI H N, DUONG NGUYEN T A, LE L P MY, et al. Power generation of Shewanella oneidensis MR-1 microbial fuel cells in bamboo fermentation effluent[J]. International Journal of Hydrogen Energy, 2021, 46(31): 16612-16621. |
38 | CHAUDHARY S, BANERJEE D K. Metal phase association of chromium in contaminated soils from an industrial area in Delhi[J]. Chemical Speciation & Bioavailability, 2004, 16(4): 145-151. |
39 | FREDRICKSON J K, ROMINE M F, BELIAEV A S, et al. Towards environmental systems biology of Shewanella [J]. Nature Reviews Microbiology, 2008, 6(8): 592-603. |
40 | LEMAIRE O N, MÉJEAN V, IOBBI-NIVOL C. The Shewanella genus: ubiquitous organisms sustaining and preserving aquatic ecosystems[J]. FEMS Microbiology Reviews, 2020, 44(2): 155-170. |
41 | FAN G, DUNDAS C M, GRAHAM A J, et al. Shewanella oneidensis as a living electrode for controlled radical polymerization[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(18): 4559-4564. |
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